爱泼斯坦-巴尔病毒BZLF1基因是从潜伏感染向裂解感染转变的开关，在B淋巴细胞初次感染后作为立即早期基因表达。
Epstein-Barr virus BZLF1 gene, a switch from latency to lytic infection, is expressed as an immediate-early gene after primary infection of B lymphocytes.
作者信息
Wen Wangrong, Iwakiri Dai, Yamamoto Koji, Maruo Seiji, Kanda Teru, Takada Kenzo
机构信息
Department of Tumor Virology, Institute for Genetic Medicine, Hokkaido University, Sapporo 060-0815, Japan.
出版信息
J Virol. 2007 Jan;81(2):1037-42. doi: 10.1128/JVI.01416-06. Epub 2006 Nov 1.
Abstract
We demonstrate here that the Epstein-Barr virus (EBV) BZLF1 gene, a switch from latent infection to lytic infection, is expressed as early as 1.5 h after EBV infection in Burkitt's lymphoma-derived, EBV-negative Akata and Daudi cells and primary B lymphocytes. Since BZLF1 mRNA is expressed even when the cells are infected with EBV in the presence of anisomycin, an inhibitor of protein synthesis, its expression does not require prerequisite protein synthesis, indicating that BZLF1 is expressed as an immediate-early gene following primary EBV infection of B lymphocytes.
摘要
我们在此证明，爱泼斯坦-巴尔病毒（EBV）的BZLF1基因，即从潜伏感染转变为裂解感染的开关，早在EBV感染伯基特淋巴瘤来源的EBV阴性Akata和Daudi细胞以及原代B淋巴细胞后的1.5小时就开始表达。由于即使在存在蛋白质合成抑制剂茴香霉素的情况下细胞被EBV感染时BZLF1 mRNA仍会表达，其表达不需要预先的蛋白质合成，这表明BZLF1是在B淋巴细胞初次感染EBV后作为立即早期基因表达的。
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