Godfrey Anja, Osborn Kay, Sinclair Alison J
School of Life Sciences, University of Sussex, Brighton BN1 9QG, UK.
Access Microbiol. 2021 Nov 26;3(11):000282. doi: 10.1099/acmi.0.000282. eCollection 2021.
Epstein-Barr virus (EBV) is present in a state of latency in infected memory B-cells and EBV-associated lymphoid and epithelial cancers. Cell stimulation or differentiation of infected B-cells and epithelial cells induces reactivation to the lytic replication cycle. In each cell type, the EBV transcription and replication factor Zta (BZLF1, EB1) plays a role in mediating the lytic cycle of EBV. Zta is a transcription factor that interacts directly with Zta response elements (ZREs) within viral and cellular genomes. Here we undertake chromatin-precipitation coupled to DNA-sequencing (ChIP-Seq) of Zta-associated DNA from cancer-derived epithelial cells. The analysis identified over 14 000 Zta-binding sites in the cellular genome. We assessed the impact of lytic cycle reactivation on changes in gene expression for a panel of Zta-associated cellular genes. Finally, we compared the Zta-binding sites identified in this study with those previously identified in B-cells and reveal substantial conservation in genes associated with Zta-binding sites.
爱泼斯坦-巴尔病毒（EBV）在受感染的记忆B细胞以及与EBV相关的淋巴和上皮癌中处于潜伏状态。受感染的B细胞和上皮细胞的细胞刺激或分化会诱导其重新激活进入裂解复制周期。在每种细胞类型中，EBV转录和复制因子Zta（BZLF1，EB1）在介导EBV的裂解周期中发挥作用。Zta是一种转录因子，可直接与病毒和细胞基因组中的Zta反应元件（ZRE）相互作用。在此，我们对来自癌症衍生上皮细胞的与Zta相关的DNA进行了染色质沉淀结合DNA测序（ChIP-Seq）。分析在细胞基因组中鉴定出超过14000个Zta结合位点。我们评估了裂解周期重新激活对一组与Zta相关的细胞基因的基因表达变化的影响。最后，我们将本研究中鉴定的Zta结合位点与先前在B细胞中鉴定的位点进行比较，发现与Zta结合位点相关的基因存在大量保守性。
