Toth C A, Thomas P
Department of Surgery, New England Deaconess Hospital, Harvard Medical School, Boston, MA 02115.
J Interferon Res. 1990 Dec;10(6):579-88. doi: 10.1089/jir.1990.10.579.
The effect of interferons (IFNs) on growth inhibition and carcinoembryonic antigen (CEA) secretion by 14 established human colorectal carcinoma cell lines was studied in vitro. The cell lines were characterized by morphologic differentiation, level of CEA production, and rate of growth. All cell lines were treated in vitro with recombinant human IFN (alpha, beta, and gamma) and the effect of treatment on growth rate and CEA secretion determined. Each cell line exhibited an individual pattern of growth inhibition that was independent of degree of differentiation, level of CEA production, and rate of growth. IFN-beta treatment did not increase CEA secretion in any of the cell lines studied. IFN-alpha and IFN-gamma resulted in increased CEA production (2- to 81-fold increase) primarily in the moderately to well-differentiated cell lines. IFN-gamma was a more potent inducer of enhanced CEA secretion than IFN-alpha. The more poorly differentiated cell lines did not produce CEA and could not be induced to do so by any of the IFNs.
体外研究了干扰素（IFN）对14种已建立的人结肠癌细胞系生长抑制及癌胚抗原（CEA）分泌的影响。这些细胞系通过形态分化、CEA产生水平和生长速率进行表征。所有细胞系均在体外接受重组人IFN（α、β和γ）处理，并测定处理对生长速率和CEA分泌的影响。每个细胞系都表现出独立于分化程度、CEA产生水平和生长速率的个体生长抑制模式。在所研究的任何细胞系中，IFN-β处理均未增加CEA分泌。IFN-α和IFN-γ主要在中度至高度分化的细胞系中导致CEA产生增加（增加2至81倍）。IFN-γ比IFN-α更有效地诱导CEA分泌增强。分化较差的细胞系不产生CEA，且任何一种IFN均不能诱导其产生CEA。
