Piggott N H, Henry J A, May F E, Westley B R
Department of Pathology, Royal Victoria Infirmary, Newcastle upon Tyne.
J Pathol. 1991 Feb;163(2):95-104. doi: 10.1002/path.1711630204.
Five peptides, corresponding to regions of the predicted protein sequence of the oestrogen-regulated pNR-2 protein which is expressed in oestrogen-responsive human breast cancer cells, were synthesized. Two peptides were immunogenic in rabbits and antisera against one peptide reacted with the pNR-2 protein in sections of formalin-fixed, paraffin-embedded breast tumour. There was a significant correlation between the extent of pNR-2 protein expression detected by immunohistochemistry and pNR-2 mRNA levels determined by hybridization with a cDNA probe in a series of primary breast tumours. pNR-2 expression was assessed immunohistochemically in a panel of normal tissues. Expression was detected in normal breast, small intestine, and stomach (body and antrum).
合成了五种肽，它们对应于雌激素调节的pNR-2蛋白预测蛋白序列的区域，该蛋白在雌激素反应性人乳腺癌细胞中表达。两种肽在兔体内具有免疫原性，针对一种肽的抗血清与福尔马林固定、石蜡包埋的乳腺肿瘤切片中的pNR-2蛋白发生反应。在一系列原发性乳腺肿瘤中，通过免疫组织化学检测到的pNR-2蛋白表达程度与通过与cDNA探针杂交测定的pNR-2 mRNA水平之间存在显著相关性。通过免疫组织化学在一组正常组织中评估pNR-2表达。在正常乳腺、小肠和胃（体部和胃窦）中检测到表达。
