Henry J A, Bennett M K, Piggott N H, Levett D L, May F E, Westley B R
Department of Pathology, University of Newcastle upon Tyne, Royal Victoria Infirmary, UK.
Br J Cancer. 1991 Oct;64(4):677-82. doi: 10.1038/bjc.1991.380.
The pNR-2/pS2 protein is regulated by oestrogens in breast cancer cell lines. This report describes a systematic survey of pNR-2/pS2 expression in a number of common epithelial tumours. Expression was evaluated immunohistochemically in an archival series using antisera raised against the C-terminus of the pNR-2/pS2 protein. Expression of pNR-2/pS2 by malignant epithelial tumours was widespread. Intense immunohistochemical staining was found in tumour cells in a proportion of pancreatic (6/8), large intestinal (7/12), gastric (9/16) and endometrial (4/12) carcinomas. Positive staining for the pNR-2/pS2 protein was also found in both benign and malignant ovarian epithelial tumours and was very significantly associated with mucinous differentiation (P less than 0.00001). Small numbers of carcinomas of bladder (2/10) and prostate (2/7) showed less intense staining and single examples of cervical carcinoma (1/7) and lung carcinoma (1/19) stained positively. None of the renal carcinomas (0/16) examined stained positively. Positive staining showed no correlation with gender. Although there are reports of oestrogen receptor expression in most of the tumour types considered, the possibility of other regulatory influences must also be considered. The pNR-2/pS2 protein may well have a more general role in human epithelial neoplasia than hitherto realised.
pNR-2/pS2蛋白在乳腺癌细胞系中受雌激素调控。本报告描述了对多种常见上皮性肿瘤中pNR-2/pS2表达的系统研究。使用针对pNR-2/pS2蛋白C末端产生的抗血清，在存档系列中通过免疫组织化学评估表达情况。恶性上皮性肿瘤中pNR-2/pS2的表达很普遍。在一部分胰腺癌（6/8）、大肠癌（7/12）、胃癌（9/16）和子宫内膜癌（4/12）的肿瘤细胞中发现了强烈的免疫组织化学染色。在良性和恶性卵巢上皮性肿瘤中也发现了pNR-2/pS2蛋白的阳性染色，并且与黏液样分化非常显著相关（P小于0.00001）。少数膀胱癌（2/10）和前列腺癌（2/7）显示出较弱的染色，而宫颈癌（1/7）和肺癌（1/19）各有1例呈阳性染色。所检查的肾癌（0/16）均无阳性染色。阳性染色与性别无关。尽管有报道称在大多数所考虑的肿瘤类型中存在雌激素受体表达，但也必须考虑其他调节影响的可能性。pNR-2/pS2蛋白在人类上皮性肿瘤中的作用可能比迄今所认识到的更为普遍。
