大麦麸阿拉伯木聚糖中存在2-O-β-D-吡喃木糖基-α-L-阿拉伯呋喃糖侧链的证据。
Evidence of the presence of 2-O-beta-D-xylopyranosyl-alpha-l-arabinofuranose side chains in barley husk arabinoxylan.
作者信息
Höije Anders, Sandström Corine, Roubroeks Johannes P, Andersson Roger, Gohil Suresh, Gatenholm Paul
机构信息
Chalmers University of Technology, Department of Chemical and Biological Engineering, Biopolymer Technology, SE-412 96 Göteborg, Sweden.
出版信息
Carbohydr Res. 2006 Dec 29;341(18):2959-66. doi: 10.1016/j.carres.2006.10.008. Epub 2006 Oct 12.
(Glucurono)arabinoxylans were extracted from barley husks and degraded with endo-beta-xylanase or subjected to periodate oxidation. The released oligosaccharide fragments were separated and isolated on Biogel-P2, and their structures were determined by NMR spectroscopy. The oligosaccharides identified consisted of beta-d-(1-->4)-linked xylopyranosyl residues, of which some were substituted at O-3 with alpha-l-arabinofuranosyl groups or at O-2 with 4-O-methylglucuronic acid. In addition to these substituents, a disaccharide side chain, 2-O-beta-d-xylopyranosyl-alpha-l-arabinofuranose, attached at position O-3 of the main chain, was proved to exist in arabinoxylan from barley husks. The compound was fully characterized with NMR, and all (1)H and (13)C NMR signals were assigned. The arabinose to xylose ratio was low (approximately 0.2) and no 2,3-disubstitution existed. No blocks of substituted xylose residues could be observed along the main chain.
（葡糖醛酸基）阿拉伯木聚糖从大麦壳中提取出来，用内切β-木聚糖酶降解或进行高碘酸盐氧化。释放出的寡糖片段在Biogel-P2上进行分离和纯化，其结构通过核磁共振光谱法测定。鉴定出的寡糖由β-D-（1→4）连接的木吡喃糖基残基组成，其中一些在O-3位被α-L-阿拉伯呋喃糖基取代，或在O-2位被4-O-甲基葡糖醛酸取代。除了这些取代基外，还证明在大麦壳阿拉伯木聚糖中存在一个连接在主链O-3位的二糖侧链，即2-O-β-D-木吡喃糖基-α-L-阿拉伯呋喃糖。该化合物通过核磁共振进行了全面表征，并对所有的¹H和¹³C核磁共振信号进行了归属。阿拉伯糖与木糖的比例较低（约为0.2），且不存在2,3-二取代。沿着主链未观察到取代木糖残基的区段。
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