Rokicki W, Rokicki M, Czyzewski K
Silesian Medical Academy, Department of Thoracosurgery, Zabrze, Poland.
Z Exp Chir Transplant Kunstliche Organe. 1990;23(3):175-9.
The study evaluated the effect of portacaval anastomosis on the regeneration ability of the liver after partial hepatectomy in rat. The regeneration of liver was evaluated using the following tests: deoxyribonucleic acid synthesis, the activity of tyrosine aminotransferase (TAT) and the concentration of DNA and RNA in subcellular fractions of liver obtained by differential centrifugation. The results indicate the harmful effect of the portacaval shunting on the liver proliferation, which was near 50% decreased and the peak of the regeneration was delayed for about 12 hours. The most significant changes were observed in the nuclear and cytoplasmic fraction. In addition, high mortality ratio was found in the animals with portacaval anastomosis followed by partial hepatectomy.
该研究评估了门腔静脉吻合术对大鼠部分肝切除术后肝脏再生能力的影响。采用以下试验评估肝脏再生情况：脱氧核糖核酸合成、酪氨酸转氨酶（TAT）活性以及通过差速离心获得的肝亚细胞组分中的DNA和RNA浓度。结果表明门腔分流对肝脏增殖有有害作用，肝脏增殖减少近50%，再生高峰延迟约12小时。在细胞核和细胞质组分中观察到最显著的变化。此外，在接受门腔静脉吻合术继而行部分肝切除术的动物中发现高死亡率。
