Kersten P J, Kopper B J, Raffa K F, Illman B L
Forest Products Laboratory, USDA Forest Service, Madison, WI 53726, USA.
J Chem Ecol. 2006 Dec;32(12):2679-85. doi: 10.1007/s10886-006-9191-z.
Diterpene resin acids are major constituents of conifer oleoresin and play important roles in tree defense against insects and microbial pathogens. The tricyclic C-20 carboxylic acids are generally classified into two groups, the abietanes and the pimaranes. The abietanes have conjugated double bonds and exhibit characteristic UV spectra. Here, we report the analysis of abietanes by reversed-phase high-performance liquid chromatography using multiwavelength detection to optimize quantification of underivatized abietic, neoabietic, palustric, levopimaric, and dehydroabietic acids. The utility of the method is demonstrated with methanol extracts of white spruce (Picea glauca) phloem, and representative concentrations are reported.
二萜树脂酸是针叶树油性树脂的主要成分，在树木抵御昆虫和微生物病原体方面发挥着重要作用。三环C-20羧酸通常分为两类，即枞酸型和海松酸型。枞酸型具有共轭双键并呈现出特征性的紫外光谱。在此，我们报告了使用多波长检测的反相高效液相色谱法对枞酸型进行分析，以优化未衍生的松香酸、新枞酸、湿地松酸、左旋海松酸和脱氢枞酸的定量分析。该方法的实用性通过白云杉（Picea glauca）韧皮部的甲醇提取物得到了验证，并报告了代表性浓度。
