Reyes-Engel Armando, Morcillo Luis, Aranda Francisco Javier, Ruiz Maximiliano, Gaitan Maria Jesús, Mayor-Olea Alvaro, Aranda Pedro, Ferrario Carlos Maria
Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Málaga, Malaga, 29080, Spain.
J Renin Angiotensin Aldosterone Syst. 2006 Jun;7(2):92-7. doi: 10.3317/jraas.2006.015.
We analysed the influence of three polymorphisms of the renin-angiotensin system (RAS) (I/D from angiotensin-converting enzyme [ACE], M235T from angiotensinogen gene [ATG] and A1166C from AT1 receptors) on plasma levels of angiotensin I (Ang I), angiotensin II (Ang II) and angiotensin-(1-7) [Ang-(1-7)].
The study population consisted of a homogeneous group of 93 healthy subjects (43 men and 50 women, mean age: 20.67+/-2.75 years). The mean blood pressure (BP) was 126+/-7/76+/-5 (SD) mmHg and the mean body mass index (BMI) was 22.4+/-2.5 kg/m2. Angiotensin peptides were separated by high performance liquid chromatography (HPLC) and quantified by radio immuno assay (RIA). Genotypes were determined by polymerase chain reaction (PCR) and restriction enzyme analysis.
Mean peptide levels were 92.48+/-102.12 pg/ml for Ang I, 22.35+/-10 pg/ml for Ang II, and 31.65+/-27.46 pg/ml for Ang-(1-7). Men had significantly higher levels of Ang-(1-7) (37.76+/-36.47 pg/ml) than women (26.04+/-13.98 pg/ml) (p<0.05). Among genotypes of each polymorphism, men with the T allele showed higher Ang- (1-7) levels compared with those with the MM genotype (p<0.05). Genotype analysis in women showed that higher Ang I levels were related with the DD genotype. When both genders were compared according to genotype, higher values of Ang-(1-7) levels and its molar ratios were found in men, and there was significantly greater Ang I levels in DD genotypes in women than men (136.72+/-112.43 vs . 65.36+/-46.83 pg/mL).
Significant correlations were found between Ang I and Ang II as well as between Ang II and Ang-(1-7) in the different study group distributions. No correlation was found between levels of Ang I and Ang-(1-7). Certain genotypes exert an influence on angiotensin peptide plasma levels which can only be seen when the population is divided according to gender.
我们分析了肾素 - 血管紧张素系统（RAS）的三种多态性（血管紧张素转换酶[ACE]的I/D、血管紧张素原基因[ATG]的M235T以及AT1受体的A1166C）对血管紧张素I（Ang I）、血管紧张素II（Ang II）和血管紧张素 -（1 - 7）[Ang -（1 - 7）]血浆水平的影响。
研究人群由93名健康受试者组成的同质群体（43名男性和50名女性，平均年龄：20.67±2.75岁）。平均血压（BP）为126±7/76±5（标准差）mmHg，平均体重指数（BMI）为22.4±2.5 kg/m²。血管紧张素肽通过高效液相色谱（HPLC）分离，并用放射免疫分析（RIA）定量。基因型通过聚合酶链反应（PCR）和限制性酶切分析确定。
Ang I的平均肽水平为92.48±102.12 pg/ml，Ang II为22.35±10 pg/ml，Ang -（1 - 7）为31.65±27.46 pg/ml。男性的Ang -（1 - 7）水平（37.76±36.47 pg/ml）显著高于女性（26.04±13.98 pg/ml）（p<0.05）。在每种多态性的基因型中，携带T等位基因的男性与MM基因型男性相比，Ang -（1 - 7）水平更高（p<0.05）。女性的基因型分析表明，较高的Ang I水平与DD基因型相关。按基因型比较男女时，发现男性的Ang -（1 - 7）水平及其摩尔比更高，并且女性DD基因型的Ang I水平显著高于男性（分别为136.72±112.43和65.36±46.83 pg/mL）。
在不同研究组分布中，发现Ang I与Ang II以及Ang II与Ang -（1 - 7）之间存在显著相关性。未发现Ang I与Ang -（1 - 7）水平之间存在相关性。某些基因型对血管紧张素肽血浆水平有影响，这种影响只有在按性别对人群进行划分时才能显现出来。
