Rajadurai M, Stanely Mainzen Prince P
Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar 608 002, Tamil Nadu, India.
Toxicology. 2006 Dec 7;228(2-3):259-68. doi: 10.1016/j.tox.2006.09.005. Epub 2006 Sep 24.
This study was designed to evaluate the cardioprotective potential of naringin on lipid peroxides, enzymatic and nonenzymatic antioxidants and histopathological findings in isoproterenol (ISO)-induced myocardial infarction (MI) in rats. Subcutaneous injection of ISO (85 mg/kg) to male Wistar rats showed a significant increase in the levels of thiobarbituric acid reactive substances and lipid hydroperoxides in plasma and the heart and a significant decrease in the activities of superoxide dismutase, catalase, glutathione peroxidase and glutathione-S-transferase in the heart and the levels of reduced glutathione, vitamin C and vitamin E in plasma and heart and ceruloplasmin in plasma. Oral administration of naringin (10, 20 and 40 mg/kg, respectively) to ISO-induced rats daily for a period of 56 days showed a significant decrease in the levels of lipid peroxidative products and improved the antioxidant status by increasing the activities of antioxidant enzymes and nonenzymatic antioxidants. Histopathological findings of the myocardial tissue showed the protective role of naringin in ISO-induced rats. The effect at a dose of 40 mg/kg of naringin was more pronounced than that of the other two doses, 10 and 20mg/kg. The results of our study show that naringin possess anti-lipoperoxidative and antioxidant activity in experimentally induced cardiac toxicity.
本研究旨在评估柚皮苷对异丙肾上腺素（ISO）诱导的大鼠心肌梗死（MI）中脂质过氧化物、酶促和非酶促抗氧化剂以及组织病理学结果的心脏保护潜力。对雄性Wistar大鼠皮下注射ISO（85mg/kg）后，血浆和心脏中硫代巴比妥酸反应性物质和脂质氢过氧化物水平显著升高，心脏中超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶和谷胱甘肽-S-转移酶的活性以及血浆和心脏中还原型谷胱甘肽、维生素C和维生素E以及血浆中铜蓝蛋白的水平显著降低。对ISO诱导的大鼠每天口服柚皮苷（分别为10、20和40mg/kg），持续56天，脂质过氧化产物水平显著降低，并通过提高抗氧化酶和非酶促抗氧化剂的活性改善了抗氧化状态。心肌组织的组织病理学结果显示柚皮苷对ISO诱导的大鼠具有保护作用。40mg/kg剂量的柚皮苷的效果比其他两个剂量（10和20mg/kg）更明显。我们的研究结果表明，柚皮苷在实验诱导的心脏毒性中具有抗脂质过氧化和抗氧化活性。
