Department of Biochemistry and Biotechnology, Annamalai University, Annamalai Nagar, Tamil Nadu 608 002, India.
Metabolism. 2010 Aug;59(8):1172-80. doi: 10.1016/j.metabol.2009.11.010. Epub 2010 Jan 4.
Myocardial infarction affects a large population in the world. Lipid peroxide metabolism plays an important role in the pathology of myocardial infarction. This study aims to evaluate the preventive effect of caffeic acid on lipid peroxides, antioxidants, cardiac marker enzymes, and histopathological findings in isoproterenol (ISO)-induced myocardial-infarcted male Wistar rats. Myocardial infarction was induced in rats by subcutaneous injection of ISO (100 mg/kg) at an interval of 24 hours for 2 days. The ISO-induced rats showed significant increase in the levels of thiobarbituric acid reactive substances, lipid hydroperoxides in the heart, plasma uric acid, and serum cardiac marker enzymes, and significant decrease in the activities of heart superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glutathione-S-transferase, and the levels of reduced glutathione, vitamin E, and vitamin C in the plasma and heart. Oral pretreatment with caffeic acid (15 mg/kg) daily for 10 days showed significant decrease in the levels of serum cardiac marker enzymes, heart lipid peroxidation products and plasma uric acid and significant increase in the levels of antioxidant system. Histopathology of myocardium also confirmed the protective effect of caffeic acid in myocardial-infarcted rats. In vitro study on total antioxidant activity (2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid assay) confirmed the strong antioxidant action of caffeic acid. Thus, the present study revealed that caffeic acid ameliorates cardiac damage in ISO-induced myocardial infarction by maintaining lipid peroxide metabolism due to its free radical scavenging and antioxidant effects. A diet containing caffeic acid may be beneficial to myocardial infarction.
心肌梗死影响世界上的大量人口。脂质过氧化物代谢在心肌梗死的病理学中起着重要作用。本研究旨在评估咖啡酸对异丙肾上腺素（ISO）诱导的心肌梗死雄性 Wistar 大鼠脂质过氧化物、抗氧化剂、心脏标志物酶和组织病理学发现的预防作用。通过皮下注射 ISO（100mg/kg），每隔 24 小时注射两次，共 2 天，诱导大鼠心肌梗死。ISO 诱导的大鼠心脏中丙二醛反应性物质、脂质过氧化物、血浆尿酸和血清心脏标志物酶水平显著升高，心脏超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶、谷胱甘肽-S-转移酶活性以及血浆和心脏中还原型谷胱甘肽、维生素 E 和维生素 C 水平显著降低。每天口服咖啡酸（15mg/kg）预处理 10 天，可显著降低血清心脏标志物酶、心脏脂质过氧化产物和血浆尿酸水平，显著增加抗氧化系统水平。心肌组织病理学也证实了咖啡酸对心肌梗死大鼠的保护作用。总抗氧化活性（2,2'-联氮双3-乙基苯并噻唑啉-6-磺酸测定法）的体外研究证实了咖啡酸的强大抗氧化作用。因此，本研究表明，咖啡酸通过清除自由基和抗氧化作用，维持脂质过氧化物代谢，从而改善 ISO 诱导的心肌梗死中的心脏损伤。含有咖啡酸的饮食可能对心肌梗死有益。
