Ranjbar Reza, Anjomruz Mehdi, Enayati Ahmad Ali, Khoobdel Mehdi, Rafinejad Atiyeh, Rafinejad Javad
Molecular Biology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
J Arthropod Borne Dis. 2020 Jun 30;14(2):126-133. doi: 10.18502/jad.v14i2.3730. eCollection 2020 Jun.
Anaplasmosis and Ehrlichiosis are the most important tick-borne diseases. This study was conducted in three cities of Kerman Province in Iran to investigate the circulation of the bacteria in ticks collected from sheep.
Ticks were collected from animals using Srkj forceps and transferred to the Entomology lab in cold chain. After specimen's identification, they kept at -70 °C. Tick DNA was extracted using Bioneers DNA extraction kits followed by Nested PCR technique to amplify ribosomal 16S rRNA gene to detect infection in ticks.
472 sheep were examined from which 349 ticks were collected and identified in laboratory using valid keys. Tick specimens belonged to two genera and four species; (62.47%) was the most frequent and (5.73%) showed the least abundance. The infestation rate to different tick species was different in three regions of Kerman Province. Observation revealed that 24 specimens (58.3%) were positive for . There is a significant difference between male and female infection rate. However, there is no significant difference between these variables in each of these cities.
This study shows high infection rates to Anaplasma in hard ticks. It is essential for health and veterinary authorities and farmers to use appropriate strategies to control ticks to reduce the infestation.
无形体病和埃立克体病是最重要的蜱传疾病。本研究在伊朗克尔曼省的三个城市开展，以调查从绵羊身上采集的蜱中这些细菌的传播情况。
使用Srkj镊子从动物身上采集蜱，并通过冷链转移至昆虫学实验室。标本鉴定后，保存在-70°C环境中。使用Bioneers DNA提取试剂盒提取蜱的DNA，随后采用巢式PCR技术扩增核糖体16S rRNA基因，以检测蜱中的感染情况。
检查了472只绵羊，从中采集了349只蜱，并在实验室使用有效分类检索表进行鉴定。蜱标本属于两个属和四个物种；（62.47%）最为常见，（5.73%）数量最少。克尔曼省三个地区对不同蜱种的感染率不同。观察发现，24个标本（58.3%）对呈阳性。雌雄感染率之间存在显著差异。然而，在这些城市中的每个城市，这些变量之间没有显著差异。
本研究表明硬蜱对无形体的感染率很高。卫生和兽医当局以及农民必须采用适当策略控制蜱虫，以减少感染。
