核糖核酸酶E是一种内切核糖核酸酶，在大肠杆菌信使核糖核酸的化学降解中起普遍作用：rne和ams是同一基因座的证据。

RNase E, an endoribonuclease, has a general role in the chemical decay of Escherichia coli mRNA: evidence that rne and ams are the same genetic locus.

作者信息

Mudd E A, Krisch H M, Higgins C F

机构信息

Imperial Cancer Research Fund Laboratories, John Radcliffe Hospital, University of Oxford, UK.

出版信息

Mol Microbiol. 1990 Dec;4(12):2127-35. doi: 10.1111/j.1365-2958.1990.tb00574.x.

DOI:10.1111/j.1365-2958.1990.tb00574.x

PMID:1708438

Abstract

Escherichia coli RNase E is known to process RNA precursors at specific sites. We show that this endoribonuclease has a general role in E. coli mRNA turnover and affects the stability of specific transcripts. The effect of the rne mutation on functional stability of mRNA was much less pronounced than that on chemical stability, although the expression of some genes was affected. The E. coli ams (altered mRNA stability) mutation was found to have phenotypes indistinguishable from those of the rne mutation, affecting both 9S RNA and T4 gene 32 mRNA processing. The rne and ams mutations were both complemented by the same 3.7 kb fragment of E. coli DNA and are probably allelic. RNase E is the first endoribonuclease identified as having a general role in the chemical decay of E. coli mRNA.

摘要

已知大肠杆菌核糖核酸酶E可在特定位点加工RNA前体。我们发现这种内切核糖核酸酶在大肠杆菌mRNA周转中起普遍作用，并影响特定转录本的稳定性。尽管某些基因的表达受到影响，但rne突变对mRNA功能稳定性的影响远不如对化学稳定性的影响明显。发现大肠杆菌ams（改变的mRNA稳定性）突变具有与rne突变无法区分的表型，影响9S RNA和T4基因32 mRNA的加工。rne和ams突变均由大肠杆菌DNA的同一3.7 kb片段互补，可能是等位基因。核糖核酸酶E是首个被鉴定在大肠杆菌mRNA化学降解中起普遍作用的内切核糖核酸酶。