Babitzke P, Kushner S R
Department of Genetics, University of Georgia, Athens 30602.
Proc Natl Acad Sci U S A. 1991 Jan 1;88(1):1-5. doi: 10.1073/pnas.88.1.1.
The in vitro and in vivo analysis of the ribonuclease E-deficient (rne-) and the altered mRNA stability protein-deficient (ams-) strains of Escherichia coli has demonstrated that they carry mutations in the same structural gene. Strains encoding either thermolabile RNase E (rne-3071) or Ams protein (ams-1) are defective in both rRNA processing and mRNA turnover. Immediately after a shift to the nonpermissive temperature, the chemical decay rate of bulk mRNA is slowed 2- to 3-fold, and within 70 min, precursors to 5S rRNA begin to accumulate. In addition, all of the phenotypes associated with either the rne-3071 or the ams-1 alleles were complemented by a recombinant plasmid carrying ams+. When taken together with previous genetic studies, these results suggest that the role of ribonuclease E in mRNA turnover involves endonucleolytic cleavages at the proposed ACAG(A/U)AUUUG consensus sequence.
对大肠杆菌核糖核酸酶E缺陷型（rne-）和改变的mRNA稳定性蛋白缺陷型（ams-）菌株进行的体外和体内分析表明，它们在同一结构基因中携带突变。编码热不稳定核糖核酸酶E（rne-3071）或Ams蛋白（ams-1）的菌株在rRNA加工和mRNA周转方面均存在缺陷。转移到非允许温度后，大量mRNA的化学衰变率立即减慢2至3倍，并且在70分钟内，5S rRNA的前体开始积累。此外，携带ams+的重组质粒互补了与rne-3071或ams-1等位基因相关的所有表型。与先前的遗传学研究结果相结合，这些结果表明核糖核酸酶E在mRNA周转中的作用涉及在所提出的ACAG(A/U)AUUUG共有序列处进行内切核酸酶切割。
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