Taraseviciene L, Miczak A, Apirion D
Department of Molecular Microbiology, Washington University School of Medicine, St Louis, Missouri 63110.
Mol Microbiol. 1991 Apr;5(4):851-5. doi: 10.1111/j.1365-2958.1991.tb00758.x.
A DNA clone complementing the rne-3071 mutation has been expressed and localized in the physical map of Escherichia coli. The DNA fragment from this clone was localized to the region of the E. coli chromosome where the rne-3071 mutation has been mapped. The position of this DNA fragment in the E. coli chromosome, the size of the product directed by this DNA fragment (110,000 Da), the restriction map of this fragment, the fact that the same clone complements the ams mutation, and the observation that the rne-3071 and the ams mutations cause similar patterns of RNA synthesis, show that the rne gene--a gene specifying the processing endonuclease RNase E--and the ams gene--a gene that affects mRNA stability--are identical.
一个能互补rne - 3071突变的DNA克隆已在大肠杆菌的物理图谱中表达并定位。来自该克隆的DNA片段被定位到大肠杆菌染色体上rne - 3071突变所在的区域。该DNA片段在大肠杆菌染色体中的位置、由该DNA片段指导合成的产物大小（110,000道尔顿）、该片段的限制性图谱、同一克隆能互补ams突变这一事实，以及rne - 3071和ams突变导致相似RNA合成模式的观察结果，表明rne基因（一个指定加工内切核酸酶RNase E的基因）和ams基因（一个影响mRNA稳定性的基因）是相同的。
