Nakase Yuen, Nakamura Tatsuo, Kin Syuichi, Nakashima Susumu, Yoshikawa Tetsuji, Kuriu Yoshiaki, Miyagawa Kouji, Sakakura Chohei, Otsuji Eigo, Ikada Yoshihito, Yamagishi Hisakazu, Hagiwara Akeo
Department of Surgery and Regenerative Medicine, Division of Surgery and Physiology of Digestive System, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, Japan.
J Surg Res. 2007 Jan;137(1):61-8. doi: 10.1016/j.jss.2006.06.019. Epub 2006 Nov 3.
The purpose of this study was to regenerate a larger size of small intestinal tissue than that of our previous study and to evaluate the regeneration of the endocrine cells (ECC) and nerve system of autologous tissue-engineered small intestine. The effect of implantation of large numbers of smooth muscle cells (SMC) for the regeneration of small intestine was also investigated.
Two types of scaffolds with different cell densities were fabricated: low density (LD) of SMC in the scaffold and high density (HD) of SMC in the scaffold. Both scaffolds were implanted into defects of isolated ileum in a canine model. Animals were sacrificed at 8, 12, 18, and 24 weeks.
The area of engineered small intestine in the HD group was four times larger than that in the LD group, although that was smaller in size than the original size of the defect. There were no significant changes in the thickness of regenerated smooth muscle layer (SML) in the LD and HD groups. The numbers of endocrine cells gradually increased after implantation. At 18 weeks of regeneration, the number of ECC reached levels comparable to that of normal mucosa. The nerve fibers extended to the center of the graft area and were observed in regenerated SML and regenerated villi at 24 weeks.
The ECC and nerve fibers were regenerated in autologous in situ tissue-engineered small intestine. Seeding a large number of SMC was not sufficient for the regeneration of the small intestine in a tubular configuration.
本研究的目的是再生比我们之前研究更大尺寸的小肠组织，并评估自体组织工程小肠内分泌细胞（ECC）和神经系统的再生情况。还研究了植入大量平滑肌细胞（SMC）对小肠再生的影响。
制备了两种具有不同细胞密度的支架：支架中SMC低密度（LD）和支架中SMC高密度（HD）。将两种支架均植入犬模型的离体回肠缺损处。在8、12、18和24周时处死动物。
HD组工程化小肠面积比LD组大4倍，尽管其尺寸小于缺损的原始大小。LD组和HD组再生平滑肌层（SML）厚度无明显变化。植入后内分泌细胞数量逐渐增加。在再生18周时，ECC数量达到与正常黏膜相当的水平。神经纤维延伸至移植区域中心，在24周时在再生的SML和再生绒毛中观察到。
自体原位组织工程小肠中ECC和神经纤维得以再生。植入大量SMC不足以使小肠以管状结构再生。
