Agascioglu Eda, Zhang Ying, Sener Abdullah, Portois Laurence, Chardigny Jean-Michel, Malaisse Willy J, Carpentier Yvon A
Laboratory of Experimental Hormonology, Brussels Free University, 808 Route de Lennik, 1070 Brussels, Belgium.
Biochimie. 2007 Mar;89(3):374-82. doi: 10.1016/j.biochi.2006.10.004. Epub 2006 Oct 30.
A depletion in long-chain polyunsaturated omega3 fatty acids may affect fuel homeostasis. In such a perspective, the present study deals mainly with the in vitro fate of D-[U-(14)C]glucose in hemidiaphragms, stretched soleus and plantaris muscle pieces obtained from normal and omega3-depleted rats (second generation) and incubated in the absence or presence of insulin. When so required, the omega3-depleted rats were injected 120 min before sacrifice with either a omega3 fatty acid-rich medium-chain triglyceride:fish oil emulsion (FO) or a control medium-chain triglyceride:olive oil emulsion (OO). The content of the soleus muscle in long-chain polyunsaturated omega3 fatty acids was severely decreased in the omega3-depleted rats, and modestly albeit significantly increased after injection of FO to these animals. In stretched soleus muscles from OO-injected omega3-depleted rats, the absolute values for glycogen synthesis measured in the absence or presence of insulin were about twice higher than in normal animals. In the OO-injected omega3-depleted rats, insulin augmented the output of (14)C-labelled amino acids, whilst such was not the case in normal animals. These and other findings suggest a lower catabolism of D-glucose relative to the anabolic process of glycogen synthesis and a lower availability of endogenous amino acids in the muscles of omega3-depleted rats, as compared to those of control animals. The prior injection of FO to the omega3-depleted rats restored a normal value for the paired ratio between the output of (14)C-labelled amino acids and acidic metabolites, but further increased glycogen net synthesis. It is proposed, therefore, that the perturbation of d-glucose metabolism in muscles from omega3-depleted rats involves a multifactorial determinism, only some of the concerned factors being susceptible to rapid correction after enrichment of cell phospholipids in long-chain polyunsaturated omega3 fatty acids.
长链多不饱和ω-3脂肪酸的缺乏可能会影响能量稳态。从这一角度来看，本研究主要探讨了D-[U-(14)C]葡萄糖在取自正常大鼠和ω-3缺乏大鼠（第二代）的半膈肌、伸展的比目鱼肌和跖肌组织块中的体外代谢情况，这些组织块在有无胰岛素的情况下进行孵育。如有需要，在处死前120分钟给ω-3缺乏大鼠注射富含ω-3脂肪酸的中链甘油三酯：鱼油乳剂（FO）或对照中链甘油三酯：橄榄油乳剂（OO）。ω-3缺乏大鼠比目鱼肌中长链多不饱和ω-3脂肪酸的含量严重降低，给这些动物注射FO后虽有增加但增幅不大。在注射OO的ω-3缺乏大鼠的伸展比目鱼肌中，无论有无胰岛素，测得的糖原合成绝对值比正常动物高出约两倍。在注射OO的ω-3缺乏大鼠中，胰岛素增加了(14)C标记氨基酸的输出量，而正常动物则不然。这些及其他发现表明，与对照动物相比，ω-3缺乏大鼠肌肉中D-葡萄糖相对于糖原合成的合成代谢过程的分解代谢较低，内源性氨基酸的可用性也较低。给ω-3缺乏大鼠预先注射FO可使(14)C标记氨基酸与酸性代谢产物输出量的配对比值恢复到正常水平，但进一步增加了糖原的净合成。因此，有人提出，ω-3缺乏大鼠肌肉中d-葡萄糖代谢的紊乱涉及多因素决定机制，只有一些相关因素在细胞磷脂中富含长链多不饱和ω-3脂肪酸后易于快速纠正。
