光皮梾木果实中的脂质过氧化、环氧化酶及肿瘤细胞增殖抑制化合物

Lipid peroxidation, cyclooxygenase enzyme and tumor cell proliferation inhibitory compounds in Cornus kousa fruits.

作者信息

Vareed Shaiju K, Schutzki Robert E, Nair Muraleedharan G

机构信息

Bioactive Natural Products and Phytoceuticals, Department of Horticulture and National Food Safety and Toxicology Center, Michigan State University, East Lansing, MI 48824, USA.

出版信息

Phytomedicine. 2007 Oct;14(10):706-9. doi: 10.1016/j.phymed.2006.09.006. Epub 2006 Nov 3.

DOI:10.1016/j.phymed.2006.09.006

PMID:17084606

Abstract

The genus Cornus is well known for its medicinal properties. Bioassay-guided isolation and characterization of C. kousa fruits afforded kaempferol 3-O-rhamnoside (1), myricetin 3-O-rhamnoside (2), kaempferol 3-O-glucoside (3), cornin (4) and stenophyllin (5) in addition to ursolic acid and beta-sitosterol. These compounds are isolated for the first time from C. kousa. Compounds 1-5 inhibited Fe(2+) catalyzed lipid peroxidation by 63%, 57%, 61%, 53%, and 51%, at 23, 22, 23, 129, and 108 microM, respectively. Similarly, they inhibited COX-1 and -2 enzymes activities by 24% and 47%, 40% and 37%, 20% and 37%, 52% and 63%, and 48% and 55% respectively, at 231, 215, 226, 258, and 217 microM, respectively. At 129 microM, compound 4 displayed growth inhibition of HCT-116 (colon), MCF-7 (breast), NCI-H460 (lung), SF-268 (central nervous system CNS), and AGS (stomach) human tumor cell lines by 31%, 29%, 40%, 9%, and 28%, respectively. Similarly, compound 5 inhibited the growth of colon, breast, lung, CNS, and stomach tumor cell lines by 0%, 27%, 35%, 16%, and 27%, respectively, at 108 microM.

摘要

山茱萸属植物以其药用特性而闻名。对四照花果实进行生物活性导向的分离和表征，除了得到熊果酸和β-谷甾醇外，还得到了山奈酚3 - O - 鼠李糖苷（1）、杨梅素3 - O - 鼠李糖苷（2）、山奈酚3 - O - 葡萄糖苷（3）、山茱萸素（4）和窄叶山茱萸素（5）。这些化合物首次从四照花中分离得到。化合物1 - 5分别在23、22、23、129和108微摩尔浓度下，对Fe(2+)催化的脂质过氧化的抑制率为63%、57%、61%、53%和51%。同样，它们分别在231、215、226、258和217微摩尔浓度下，对COX - 1和 - 2酶活性的抑制率分别为24%和47%、40%和37%、20%和37%、52%和63%、48%和55%。在129微摩尔浓度下，化合物4对HCT - 116（结肠）、MCF - 7（乳腺）、NCI - H460（肺）、SF - 268（中枢神经系统CNS）和AGS（胃）人肿瘤细胞系的生长抑制率分别为31%、29%、40%、9%和28%。同样，化合物5在108微摩尔浓度下，对结肠、乳腺、肺、CNS和胃肿瘤细胞系的生长抑制率分别为0%、27%、35%、16%和27%。