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从[植物名称]的茎和茎皮中提取的水杨酸苄酯作为一种肾保护剂，可对抗顺铂诱导的LLC-PK1细胞凋亡性细胞死亡。

Benzyl salicylate from the stems and stem barks of as a nephroprotective agent against cisplatin-induced apoptotic cell death in LLC-PK1 cells.

作者信息

Lee Dahae, Lee Seoung Rak, Kang Ki Sung, Kim Ki Hyun

机构信息

School of Pharmacy, Sungkyunkwan University Suwon 440-746 Republic of Korea

College of Korean Medicine, Gachon University Seongnam 13120 Republic of Korea

出版信息

RSC Adv. 2020 Feb 5;10(10):5777-5784. doi: 10.1039/c9ra07009e. eCollection 2020 Feb 4.

DOI:10.1039/c9ra07009e
PMID:35497438
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9049590/
Abstract

Nephrotoxicity currently plagues the therapeutic use of cisplatin. Its major side effects are predominantly associated with renal tubular cell apoptosis. In this study, we examined the potential nephroprotective constituents from that can exert effects against cisplatin-induced oxidative stress and apoptosis in renal tubular epithelial (LLC-PK1) cells. Phytochemical investigation of the MeOH extract of the stems and stem barks of led to the isolation of the potential nephroprotective constituents (1-15). Among the isolates, treatment with benzyl salicylate (BS) (15) (50 and 100 μM) significantly attenuated LLC-PK1 cell apoptosis and potently blocked cisplatin-induced nuclei condensation, intracellular ROS generation, and apoptosis, as indicated by Hoechst 33342 and HDCFDA staining. Furthermore, the expression of apoptosis-related proteins such as MAPK, Bax/Bcl-2 protein ratio, and cleaved caspase-9 and -3, were significantly decreased by BS. These findings provide that benzyl salicylate can be a nephroprotective agent against cisplatin-induced oxidative stress and apoptosis.

摘要

肾毒性目前困扰着顺铂的治疗应用。其主要副作用主要与肾小管细胞凋亡有关。在本研究中，我们研究了[植物名称]中可能具有肾保护作用的成分，这些成分可以对顺铂诱导的肾小管上皮（LLC-PK1）细胞氧化应激和凋亡产生影响。对[植物名称]茎和茎皮的甲醇提取物进行植物化学研究，分离出了可能具有肾保护作用的成分（1-15）。在这些分离物中，用乙酰水杨酸苄酯（BS）（15）（50和100μM）处理可显著减轻LLC-PK1细胞凋亡，并有效阻断顺铂诱导的细胞核浓缩、细胞内活性氧生成和凋亡，Hoechst 33342和HDCFDA染色表明了这一点。此外，BS显著降低了凋亡相关蛋白如MAPK、Bax/Bcl-2蛋白比率以及裂解的caspase-9和-3的表达。这些发现表明，乙酰水杨酸苄酯可以作为一种抗顺铂诱导的氧化应激和凋亡的肾保护剂。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/607a/9049590/e61d60df1c53/c9ra07009e-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/607a/9049590/a3f4ed695a91/c9ra07009e-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/607a/9049590/8e6456d81d99/c9ra07009e-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/607a/9049590/3c4e5731492c/c9ra07009e-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/607a/9049590/63173e650a22/c9ra07009e-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/607a/9049590/c078543b3a84/c9ra07009e-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/607a/9049590/e61d60df1c53/c9ra07009e-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/607a/9049590/a3f4ed695a91/c9ra07009e-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/607a/9049590/8e6456d81d99/c9ra07009e-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/607a/9049590/3c4e5731492c/c9ra07009e-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/607a/9049590/63173e650a22/c9ra07009e-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/607a/9049590/c078543b3a84/c9ra07009e-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/607a/9049590/e61d60df1c53/c9ra07009e-f6.jpg

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