Barliana Melisa I, Suradji Eka W, Abdulah Rizky, Diantini Ajeng, Hatabu Toshimitsu, Nakajima-Shimada Junko, Subarnas Anas, Koyama Hiroshi
Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor, West Java 45363, Indonesia.
Department of Health, Teluk Bintuni Regency, West Papua 98364, Indonesia ; Department of Public Health, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan.
Biomed Rep. 2014 Jul;2(4):579-583. doi: 10.3892/br.2014.271. Epub 2014 Apr 28.
Previous intervention studies have shown that the most effective agents used in the treatment of malaria were isolated from natural sources. Plants consumed by non-human primates serve as potential drug sources for human disease management due to the similarities in anatomy, physiology and disease characteristics. The present study investigated the antiplasmodial properties of the primate-consumed plant, ( Korth. (family ), which has already been reported to have several biological activities. The ethanol extract of was fractionated based on polarity using -hexane, ethyl acetate and water. The antiplasmodial activity was tested against chloroquine-resistant () at 100 μg/ml for 72 h. The major compound of the most active ethyl acetate fraction was subsequently isolated using column chromatography and identified by nuclear magnetic resonance. The characterized compound was also tested against chloroquine-resistant in culture to evaluate its antiplasmodial activity. The ethanol extract of at 100 μg/ml exhibited a significant parasite shrinkage after 24 h of treatment. The ethyl acetate fraction at 100 μg/ml was the most active fraction against chloroquine-resistant . Based on the structural characterization, the major compound isolated from the ethyl acetate fraction was kaempferol-3--rhamnoside, which showed promising antiplasmodial activity against chloroquine-resistant with an IC of 106 μM after 24 h of treatment. The present study has provided a basis for the further investigation of kaempferol-3--rhamnoside as an active compound for potential antimalarial therapeutics.
先前的干预研究表明，用于治疗疟疾的最有效药物是从天然来源分离得到的。由于非人类灵长类动物在解剖学、生理学和疾病特征方面存在相似性，它们食用的植物可作为人类疾病管理的潜在药物来源。本研究调查了灵长类动物食用的植物（科特氏 ，科 ）的抗疟特性，该植物已被报道具有多种生物活性。基于极性，使用正己烷、乙酸乙酯和水对 的乙醇提取物进行了分级分离。在100 μg/ml的浓度下，针对耐氯喹疟原虫（ ）测试了抗疟活性，持续72小时。随后，使用柱色谱法分离出最具活性的乙酸乙酯级分中的主要化合物，并通过核磁共振进行鉴定。还在培养物中针对耐氯喹疟原虫测试了所鉴定的化合物，以评估其抗疟活性。在100 μg/ml浓度下， 的乙醇提取物在处理24小时后显示出明显的寄生虫萎缩。在100 μg/ml浓度下，乙酸乙酯级分是针对耐氯喹疟原虫最具活性的级分。基于结构表征，从乙酸乙酯级分中分离出的主要化合物是山奈酚-3-鼠李糖苷，在处理24小时后，其对耐氯喹疟原虫显示出有前景的抗疟活性，IC50为106 μM。本研究为进一步研究山奈酚-3-鼠李糖苷作为潜在抗疟治疗的活性化合物提供了基础。
