Graybiel A M, Chesselet M F
Proc Natl Acad Sci U S A. 1984 Dec;81(24):7980-4. doi: 10.1073/pnas.81.24.7980.
Striatal cell bodies and fibers expressing [Met]enkephalin [( Met]Enk)-like immunoreactivity were studied with two variants of the peroxidase-antiperoxidase method in normal primates and cats and in cats pretreated with colchicine. Strikingly different patterns of [Met]Enk-like immunoreactivity were observed, both in fiber and cell body immunostaining, depending on the technical protocols followed; no single histochemical protocol fully revealed the compartmentalization present. In the dorsal striatum, patches of [Met]Enk-positive neuropil, known to line up with the acetylcholinesterase-poor striatal zones called striosomes, appeared in sections treated by protocols favoring fiber immunostaining. In sections stained by procedures favoring perikaryal staining, the striosomes appeared as Enk-poor patches in a field of immunoreactive cells and neuropil. When cell-body staining was enhanced by pretreatment with colchicine, cells expressing [Met]Enk-like immunoreactivity appeared both in and out of striosomes, and the striosomal neuropil appeared Enk-rich. These results suggest that there are subtypes of Enk-positive neurons in the striatum, including a "colchicine-dependent subtype" in dorsal striosomes, and suggest that the Enk-positive striatal neuropil is also made up of different components. Immunospecificity of this dorsal striosomal system was further demonstrated by the finding that neurons expressing intense immunoreactivity to substance P and to dynorphin B were largely confined to striosomes.
采用过氧化物酶-抗过氧化物酶方法的两种变体，对正常灵长类动物和猫以及用秋水仙碱预处理的猫中表达[甲硫氨酸]脑啡肽（[Met]Enk）样免疫反应性的纹状体细胞体和纤维进行了研究。根据所采用的技术方案，在纤维和细胞体免疫染色中均观察到了显著不同的[Met]Enk样免疫反应性模式；没有一种单一的组织化学方案能完全揭示其存在的分区情况。在背侧纹状体中，[Met]Enk阳性神经毡斑块，已知与称为纹状体小体的乙酰胆碱酯酶缺乏的纹状体区域排列一致，出现在有利于纤维免疫染色的方案处理的切片中。在有利于核周染色的程序染色的切片中，纹状体小体在免疫反应性细胞和神经毡的区域中表现为Enk缺乏的斑块。当通过秋水仙碱预处理增强细胞体染色时，表达[Met]Enk样免疫反应性的细胞出现在纹状体小体内外，并且纹状体小体神经毡显得Enk丰富。这些结果表明纹状体中存在Enk阳性神经元的亚型，包括背侧纹状体小体中的“秋水仙碱依赖性亚型”，并表明Enk阳性纹状体神经毡也由不同成分组成。对P物质和强啡肽B表达强烈免疫反应性的神经元主要局限于纹状体小体这一发现，进一步证明了这个背侧纹状体小体系统的免疫特异性。
