猪的横纹肌尿道括约肌展现出了对于评估括约肌功能不全治疗方法至关重要的形态学和功能特征。

The striated urethral sphincter of the pig shows morphological and functional characteristics essential for the evaluation of treatments for sphincter insufficiency.

作者信息

Zini Laurent, Lecoeur Constant, Swieb Salem, Combrisson Hélène, Delmas Vincent, Ghérardi Romain, Abbou Claude, Chopin Dominique, Yiou René

机构信息

Service d'Urologie, Centre Hospitalier Universitaire Henri Mondor, 51 avenue du Maréchal de Lattre de Tassigny, 94010 Créteil, France.

出版信息

J Urol. 2006 Dec;176(6 Pt 1):2729-35. doi: 10.1016/j.juro.2006.07.135.

DOI:10.1016/j.juro.2006.07.135

PMID:17085204

Abstract

PURPOSE

New treatments are currently under investigation for intrinsic sphincter insufficiency. However, animal models in which to study the resting urethral tone generated by the striated urethral sphincter are still lacking. We describe the striated urethral sphincter in the pig. We investigated its participation in resting urethral tone with the aim of developing new tools for evaluating therapies for sphincter insufficiency.

MATERIALS AND METHODS

A total of 15 female pigs were used in this study. Anatomy of the striated urethral sphincter was described via transpubic and endoscopic approaches. Participation of the striated urethral sphincter in resting urethral tone was assessed by analysis of urethral pressure profilometry (maximum urethral closure pressure, functional urethral length and area under the curve) before and after curare injection or by destruction of the striated urethral sphincter by endoscopic electrocautery. Serial urethral cross sections were immunostained for slow/fast myosin and digitalized for 3-dimensional reconstructions to determine striated urethral sphincter volume.

RESULTS

The striated urethral sphincter was Omega-shaped and it encircled the distal third of the urethra. A mean peak intraurethral pressure +/- SEM of 58.9 +/- 13.4 cm H(2)O was noted in front of the striated urethral sphincter. Curare injection and endoscopic injury decreased maximum urethral closure pressure by 48.4% and 51.1%, functional urethral length by 10.3% and 15.3%, and area under the curve by 47% and 64%, respectively. The striated urethral sphincter consisted of 52% slow and 48% fast myofibers. Its mean volume was 0.87 cm.

CONCLUSIONS

The striated urethral sphincter of the female pig shows the morphological and functional features of a tonic muscle. Methods of measuring resting urethral tone generated by the striated urethral sphincter represent original tools for evaluating therapies for intrinsic sphincter insufficiency.

摘要

目的

目前正在研究针对固有括约肌功能不全的新治疗方法。然而，仍缺乏用于研究横纹肌尿道括约肌产生的静息尿道张力的动物模型。我们描述了猪的横纹肌尿道括约肌。我们研究了其在静息尿道张力中的作用，旨在开发评估括约肌功能不全治疗方法的新工具。

材料与方法

本研究共使用了15只雌性猪。通过经耻骨和内镜方法描述横纹肌尿道括约肌的解剖结构。通过分析箭毒注射前后或内镜电灼破坏横纹肌尿道括约肌前后的尿道压力轮廓图（最大尿道闭合压、功能性尿道长度和曲线下面积）来评估横纹肌尿道括约肌在静息尿道张力中的作用。对连续的尿道横截面进行慢/快肌球蛋白免疫染色并数字化以进行三维重建，以确定横纹肌尿道括约肌的体积。

结果

横纹肌尿道括约肌呈Ω形，环绕尿道远端三分之一。在横纹肌尿道括约肌前方测得平均尿道内压峰值±标准误为58.9±13.4 cm H₂O。箭毒注射和内镜损伤分别使最大尿道闭合压降低48.4%和51.1%，功能性尿道长度降低10.3%和15.3%，曲线下面积降低47%和64%。横纹肌尿道括约肌由52%的慢肌纤维和48%的快肌纤维组成。其平均体积为0.87 cm³。