Moon H-I, Lee J, Chung J H
Molecular Neuroscience and Vascular Biology Laboratory, Department of Surgery, University of Kentucky Medical Center, Lexington, Kentucky 40536, USA.
Phytomedicine. 2006 Nov;13(9-10):707-11. doi: 10.1016/j.phymed.2005.10.001. Epub 2005 Nov 15.
Methanol and aqueous extracts of Styrax japonica used traditionally for the treatments of skin elastic materials were screened in vitro for the matrix metalloproteinase (MMP)-1 inhibitor actions. The methylene chloride soluble fraction of methanol extract from the stems of S. japonica showed significant MMP-1 inhibition in primary old aged human skin fibroblasts caused by ultraviolet (UV) irradiation. Main triterpenoids were isolated by repeated column chromatography. Among them, the triterpenoid erythrodiol-3-acetate reduced the expression of MMP-1 and induced the expression of type-1 procollagen at the protein levels in a dose-dependent manner caused by UV irradiated cultured old aged human skin fibroblasts. Taken together, our results suggest that erythrodiol-3-acetate plays an important role in the skin aging process caused by UV irradiation.
传统上用于治疗皮肤弹性材料的日本安息香的甲醇提取物和水提取物，在体外进行了基质金属蛋白酶（MMP）-1抑制剂作用的筛选。日本安息香茎部甲醇提取物的二氯甲烷可溶部分在紫外线（UV）照射引起的原代老年人类皮肤成纤维细胞中显示出显著的MMP-1抑制作用。通过反复柱色谱法分离出主要的三萜类化合物。其中，三萜类化合物赤藓醇-3-乙酸酯在紫外线照射培养的老年人类皮肤成纤维细胞引起的蛋白水平上，以剂量依赖的方式降低了MMP-1的表达并诱导了I型前胶原的表达。综上所述，我们的结果表明赤藓醇-3-乙酸酯在紫外线照射引起的皮肤衰老过程中起重要作用。
