Wang Z L, Sun J Y, Wang D N, Xie Y H, Wang S W, Zhao W M
School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
Phytomedicine. 2006 Nov;13(9-10):718-23. doi: 10.1016/j.phymed.2005.09.005. Epub 2005 Oct 27.
In this report, we used genistein that was extracted from a Chinese herbal medicine Huaijiao (Sophora japonica-Leguminosae) to evaluate its pharmacological function on anti-osteoporosis. This genistein is purified in a large-scale production from Huaijiao by a state-of-art method as described by Tian et al. [2004. The preparation of genistein and LC-MS/MS on-line analysis. Drug Devel. Res. 61, 6-12]. Chemical structure of the isolated genistein was examined by using various techniques including nuclear magnetic resonant spectrum, infrared absorption spectrum, ultraviolet absorption spectrum and mass spectrum, and was proved to be identical to those purified from soybean in a small scale as previously reported. We randomly divided female SD rats into 6 groups, including control, ovariectomized model, Nilestriol-treated, and three level of dosages of genistein-treated. We evaluated the pharmacological effects of genistein against osteoporosis by measuring the bone density of femur and bone mineral group including calcium, phosphorous, and magnesium. The consequences of genistein treatment on bone histology and morphology were also determined by measuring the trabcular area, thickness and number. Our results indicated that treatment with a 4.5 or 9 mg/kg dosage of genistein could also prevent osteoporosis significantly at the 4th week after treatment. In comparison with the anti-osteoporosis effects of soybean genistein, the genistein extracted from Huaijiao has the same beneficial effect on anti-osteoporosis.
在本报告中，我们使用从中药槐角（豆科槐属植物）中提取的染料木黄酮来评估其抗骨质疏松的药理作用。这种染料木黄酮是按照Tian等人[2004年。染料木黄酮的制备及液相色谱-串联质谱在线分析。药物研发。61，6 - 12]所述的先进方法从槐角中大规模纯化得到的。通过使用包括核磁共振谱、红外吸收光谱、紫外吸收光谱和质谱等各种技术对分离得到的染料木黄酮的化学结构进行了检测，结果证明其与先前报道的从大豆中小规模纯化得到的染料木黄酮结构相同。我们将雌性SD大鼠随机分为6组，包括对照组、去卵巢模型组、尼尔雌醇治疗组以及三个不同剂量的染料木黄酮治疗组。我们通过测量股骨骨密度以及骨矿物质（包括钙、磷和镁）来评估染料木黄酮抗骨质疏松的药理作用。还通过测量骨小梁面积、厚度和数量来确定染料木黄酮治疗对骨组织学和形态学的影响。我们的结果表明，在治疗后第4周，给予4.5或9 mg/kg剂量的染料木黄酮治疗也能显著预防骨质疏松。与大豆染料木黄酮的抗骨质疏松作用相比，从槐角中提取的染料木黄酮在抗骨质疏松方面具有相同的有益效果。
