Morrison W I, Howard C J, Hinson C A
ILRAD, Nairobi, Kenya.
Vet Immunol Immunopathol. 1991 Jan;27(1-3):235-8. doi: 10.1016/0165-2427(91)90106-m.
Monoclonal antibodies (mAbs) specific for bovine CD4 and CD5 antigens have been found to identify polymorphic determinants on these molecules. In the case of CD5, mAb IL-A67 recognises one allotypic form of the antigen while four other CD5-specific mAbs in the workshop (CC17, CC29, BLT-1 and 8C11) recognise a second allotype. The CD4-specific mAbs submitted to the workshop reacted with the cells of all animals tested. However, a further two mAbs (CC26 and IL-A18) specific for CD4 were found to react with cells only from about 85% of animals tested. Sequential immuno-precipitation experiments together with family studies showed that the allotypes of CD4 and CD5 are both inherited in a simple Mendelian manner and are co-dominantly expressed. One of the CD5 allotypes was not detected in Bos taurus animals while the gene frequency of the second allotype was only about 10% in the B. indicus animals tested. The gene frequency of the CD4 allotype detected by CC26 and IL-A18 was similar in the two sub-species.
已发现针对牛CD4和CD5抗原的单克隆抗体（mAb）可识别这些分子上的多态性决定簇。就CD5而言，单克隆抗体IL-A67识别该抗原的一种同种异型形式，而研讨会上的其他四种CD5特异性单克隆抗体（CC17、CC29、BLT-1和8C11）识别另一种同种异型。提交给研讨会的CD4特异性单克隆抗体与所有测试动物的细胞发生反应。然而，发现另外两种针对CD4的单克隆抗体（CC26和IL-A18）仅与约85%的测试动物的细胞发生反应。连续免疫沉淀实验以及家系研究表明，CD4和CD5的同种异型均以简单的孟德尔方式遗传且共显性表达。在瘤牛动物中未检测到一种CD5同种异型，而在测试的印度瘤牛动物中，第二种同种异型的基因频率仅约为10%。CC26和IL-A18检测到的CD4同种异型的基因频率在两个亚种中相似。
