Morrison W I, Davis W C
IAH, Compton, Newbury, UK.
Vet Immunol Immunopathol. 1991 Jan;27(1-3):71-6. doi: 10.1016/0165-2427(91)90082-n.
The 14 mAbs representing workshop cluster 1 recognise a 215/300 kDa antigen expressed on a subpopulation of lymphocytes which express low levels of CD5 but are negative for other B and T cell markers defined by workshop antibodies. Separate studies with cDNA probes for bovine CD3 and T cell receptor indicate that these lymphocytes are gamma/delta T cells. It is of note that the different mAbs react with varying proportions of this cell population, suggesting that the antigen undergoes considerable post-translational modification. A further two mAbs, designated workshop cluster 2, react with a 37/47 kDa heterodimeric molecule expressed in a subpopulation of the WC1+ cells and on an additional small population of T lymphocytes. The cell populations recognised by the two mAbs are different although they overlap in some animals. It is suggested that these mAbs may be specific for T cell receptor molecules.
代表研讨会集群1的14种单克隆抗体识别一种在淋巴细胞亚群上表达的215/300 kDa抗原，这些淋巴细胞表达低水平的CD5，但对研讨会抗体定义的其他B细胞和T细胞标志物呈阴性。用牛CD3和T细胞受体的cDNA探针进行的单独研究表明，这些淋巴细胞是γ/δ T细胞。值得注意的是，不同的单克隆抗体与该细胞群体的不同比例发生反应，这表明该抗原经历了大量的翻译后修饰。另外两种单克隆抗体，称为研讨会集群2，与在WC1+细胞亚群和另外一小部分T淋巴细胞上表达的37/47 kDa异二聚体分子发生反应。这两种单克隆抗体识别的细胞群体不同，尽管它们在一些动物中存在重叠。有人认为这些单克隆抗体可能对T细胞受体分子具有特异性。
