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成年鸡盲肠中产甲烷古菌的鉴定与定量分析。

Identification and quantification of methanogenic Archaea in adult chicken ceca.

作者信息

Saengkerdsub Suwat, Anderson Robin C, Wilkinson Heather H, Kim Woo-Kyun, Nisbet David J, Ricke Steven C

机构信息

University of Arkansas, Department of Food Science, 2650 N. Young Avenue, Fayetteville, AR 72704-5690, USA.

出版信息

Appl Environ Microbiol. 2007 Jan;73(1):353-6. doi: 10.1128/AEM.01931-06. Epub 2006 Nov 3.

Abstract

By using molecular methods for the identification and quantification of methanogenic archaea in adult chicken ceca, 16S rRNA genes of 11 different phylotypes, 10 of which were 99% similar to Methanobrevibacter woesei, were found. Methanogen populations, as assessed by cultivation, and the 16S rRNA copy number were between 6.38 and 8.23 cells/g (wet weight) and 5.50 and 7.19 log(10)/g (wet weight), respectively.

摘要

通过使用分子方法对成年鸡盲肠中产甲烷古菌进行鉴定和定量,发现了11种不同系统发育型的16S rRNA基因,其中10种与沃氏甲烷短杆菌的相似度为99%。通过培养评估的产甲烷菌数量和16S rRNA拷贝数分别在6.38至8.23个细胞/克(湿重)和5.50至7.19 log(10)/克(湿重)之间。

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