Animal Science Department, Biotechnical Faculty, University of Ljubljana, Chair for Microbiology and Microbial Biotechnology, Domžale, Slovenia.
FEMS Microbiol Ecol. 2010 Dec;74(3):623-30. doi: 10.1111/j.1574-6941.2010.00980.x. Epub 2010 Oct 15.
During a comparison of 16S rDNA PCR-denaturant gradient gel electrophoresis (DGGE) profiles of methanogenic archaea from rumen fluid, rabbit caecum and pig feces, a unique band common to all rabbit caecum samples was observed. DGGE profiling also showed that the methanogen community from the New Zealand White adult rabbits is different and less complex than the methanogen communities from the rumen and pig feces. Small subunit ribosomal gene sequences of methanogenic archaea were subsequently retrieved from the constructed rabbit caecum 16S rDNA gene library. Results of the phylogenetic analysis indicated that rabbit caecum is inhabited by members of the genus Methanobrevibacter and is possibly one-species dominated, because all the retrieved sequences exhibited similarity values of 99% or higher. This species may well be a novel species of the genus Methanobrevibacter. It belongs to a distinct phylogenetic group containing Methanobrevibacter woesei, Methanobrevibacter thaueri and Methanobrevibacter gottschalkii strains isolated from animal feces, and Methanobrevibacter smithii from the predominating methanogen population of the human large bowel.
在对来自瘤胃液、兔盲肠和猪粪便的产甲烷古菌 16S rDNA PCR-变性梯度凝胶电泳（DGGE）图谱进行比较时，观察到所有兔盲肠样本中都存在一条共同的独特条带。DGGE 分析还表明，新西兰白兔盲肠中的产甲烷菌群落与瘤胃和猪粪便中的产甲烷菌群落不同，且更为简单。随后，从构建的兔盲肠 16S rDNA 基因文库中检索到产甲烷古菌的小亚基核糖体基因序列。系统发育分析结果表明，兔盲肠中栖息着甲烷短杆菌属的成员，可能是单一种群占主导地位，因为所有检索到的序列相似度值均为 99%或更高。该物种很可能是甲烷短杆菌属的一个新种。它属于一个独特的进化群，其中包含来自动物粪便的甲烷短杆菌、甲烷短杆菌和甲烷短杆菌菌株，以及来自人大肠中主要产甲烷菌群体的甲烷短杆菌史密斯。
