is a common foodborne pathogen found in poultry that can cause severe life-threatening illnesses in humans. It is important to detect this pathogen in food to manage foodborne outbreaks. This study reports a novel impedimetric phage protein-based biosensor to detect NCTC 11168 at 100 CFU/mL concentrations using a genetically engineered receptor-binding phage protein, FlaGrab, as a bioreceptor. The electrochemical impedance spectroscopy (EIS) technique was employed to measure changes in resistance upon interaction with . The sensitivity of the phage protein-immobilized electrode was assessed using the various concentrations of NCTC 11168 ranging from 10-10 colony forming units (CFU)/mL). The change transfer resistance of the biosensor increased with increasing numbers of NCTC 11168 cells. The detection limit was determined to be approximately 10 CFU/mL in the buffer and 10 CFU/mL in the ex vivo samples. subsp. serotype Typhimurium-291RH and Scott A were used as nontarget bacterial cells to assess the specificity of the developed biosensor. Results showed that the developed biosensor was highly specific toward the target NCTC 11168, as no signal was observed for the nontarget bacterial cells.