Nano Electrochemistry Laboratory, School of Chemical, Materials and Biomedical Engineering, University of Georgia, Athens, GA 30602, USA.
Department of Microbiology, University of Georgia, Athens, GA 30602, USA.
Biosensors (Basel). 2024 Aug 21;14(8):402. doi: 10.3390/bios14080402.
is a common foodborne pathogen found in poultry that can cause severe life-threatening illnesses in humans. It is important to detect this pathogen in food to manage foodborne outbreaks. This study reports a novel impedimetric phage protein-based biosensor to detect NCTC 11168 at 100 CFU/mL concentrations using a genetically engineered receptor-binding phage protein, FlaGrab, as a bioreceptor. The electrochemical impedance spectroscopy (EIS) technique was employed to measure changes in resistance upon interaction with . The sensitivity of the phage protein-immobilized electrode was assessed using the various concentrations of NCTC 11168 ranging from 10-10 colony forming units (CFU)/mL). The change transfer resistance of the biosensor increased with increasing numbers of NCTC 11168 cells. The detection limit was determined to be approximately 10 CFU/mL in the buffer and 10 CFU/mL in the ex vivo samples. subsp. serotype Typhimurium-291RH and Scott A were used as nontarget bacterial cells to assess the specificity of the developed biosensor. Results showed that the developed biosensor was highly specific toward the target NCTC 11168, as no signal was observed for the nontarget bacterial cells.
是一种常见的食源性病原体，存在于家禽中，可导致人类严重的危及生命的疾病。在食品中检测这种病原体对于管理食源性疫情非常重要。本研究报告了一种新型基于噬菌体蛋白的阻抗生物传感器，该传感器使用经过基因工程改造的受体结合噬菌体蛋白 FlaGrab 作为生物受体，可在 100 CFU/mL 浓度下检测到 NCTC 11168。电化学阻抗谱 (EIS) 技术用于测量与相互作用时电阻的变化。使用不同浓度的 NCTC 11168（范围为 10-10 个菌落形成单位 (CFU)/mL）评估噬菌体蛋白固定电极的灵敏度。生物传感器的转移电阻随 NCTC 11168 细胞数量的增加而增加。在缓冲液中的检测限约为 10 CFU/mL，在离体样本中的检测限约为 10 CFU/mL。副伤寒沙门氏菌血清型 Typhimurium-291RH 和 Scott A 被用作非靶标细菌细胞，以评估所开发生物传感器的特异性。结果表明，该生物传感器对目标 NCTC 11168 具有高度特异性，因为未观察到非靶标细菌细胞的信号。
