[乙型肝炎病毒前S1抗原、前S2抗原、大蛋白、前S2抗体检测的意义及对HBV DNA复制的预测。]
[Significance of hepatitis B virus PreS1-Ag, PreS2-Ag, large protein, PreS2-Ab detection and the prediction of HBV DNA replication.].
作者信息
Mao Y L, Li B A, Ma H B, Sun Z Q, Shi J B, Li X H, Xu J, Wang X F, Yang L H
机构信息
Center of Clinical Laboratory, The No. 302 Hospital of The People's Liberation Army, Beijing 100039, China.
出版信息
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2006 Sep;20(3):276-8.
BACKGROUND
To explore the significance of hepatitis B virus PreS1-Ag, PreS2-Ag, large protein (LP) detection and the prediction of viral replication.
METHODS
PreS1-Ag, PreS2-Ag, LP and HBV markers were measured by enzyme linked immunosorbent assay (ELISA) in 201 cases of infected serum. Serum HBV DNA level was quantitatively detected by real-time polymerase chain reaction (PCR).
RESULTS
There were significant differences in positive rate between the PreS1-Ag, PreS2-Ag, LP, and HBsAg; the positive rate of PreS2-Ag and LP were higher than that of the HBeAg. No significant differences were found in the positive rates between LP and the levels of HBV DNA and there was a positive correlation between quantitations of HBV DNA and HBV-LP.
CONCLUSION
Serum PreS1-Ag, PreS2-Ag and LP were laboratory markers that can accurately reflect HBV DNA reproduction, and were helpful complementarity to traditional HBV M. There is a close correlation between the number of copies of HBV DNA and the levels of HBV-LP.
背景
探讨乙型肝炎病毒前S1抗原、前S2抗原、大蛋白（LP）检测的意义及对病毒复制的预测。
方法
采用酶联免疫吸附试验（ELISA）检测201例感染血清中的前S1抗原、前S2抗原、LP及乙肝病毒标志物。采用实时聚合酶链反应（PCR）定量检测血清乙肝病毒DNA水平。
结果
前S1抗原、前S2抗原、LP与乙肝表面抗原（HBsAg）的阳性率存在显著差异；前S2抗原和LP的阳性率高于e抗原（HBeAg）。LP的阳性率与乙肝病毒DNA水平之间无显著差异，乙肝病毒DNA定量与乙肝病毒-LP之间呈正相关。
结论
血清前S1抗原、前S2抗原和LP是能准确反映乙肝病毒DNA复制的实验室指标，对传统乙肝病毒标志物有辅助补充作用。乙肝病毒DNA拷贝数与乙肝病毒-LP水平密切相关。
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