van de Graaf E A, Jansen H M, Weber J A, Koolen M G, Out T A
Department of Pulmonology, Academic Medical Centre, Amsterdam, The Netherlands.
Clin Chim Acta. 1991 Jan 31;196(1):27-39. doi: 10.1016/0009-8981(91)90205-q.
In 6 healthy controls and 23 patients with pulmonary diseases the influx of urea during bronchoalveolar lavage was measured by comparing the concentrations of albumin and urea in the sequential samples recovered. It varied between -28 and 151 mumol/l. In the bronchoalveolar lavage fluid and serum we measured alpha-2-macroglobulin (A2M) and ceruloplasmin (CP). The bronchoalveolar lavage fluid to serum ratios were calculated (QCP and QA2M). QA2M/QCP was taken as a measure of the respiratory membrane permeability; it varied between 0.05 and 0.53. Influx of urea during lavage was higher according as the QA2M/QCP ratio was higher. We conclude that concentrations of substances in the epithelial lining fluid calculated with the urea correction method have to be corrected for the influx of urea.
在6名健康对照者和23例肺部疾病患者中，通过比较回收的连续样本中白蛋白和尿素的浓度，测量支气管肺泡灌洗期间尿素的流入量。其变化范围在-28至151μmol/L之间。在支气管肺泡灌洗液和血清中，我们测量了α-2-巨球蛋白（A2M）和铜蓝蛋白（CP）。计算支气管肺泡灌洗液与血清的比值（QCP和QA2M）。将QA2M/QCP作为呼吸膜通透性的指标；其变化范围在0.05至0.53之间。灌洗期间尿素的流入量随着QA2M/QCP比值的升高而增加。我们得出结论，用尿素校正法计算的上皮衬液中物质的浓度必须针对尿素的流入量进行校正。
