Cheong S K, Lim Y C
Division of Haematology, Faculty of Medicine, National University of Malaysia, Kuala Lumpur.
Malays J Pathol. 1990 Jun;12(1):51-6.
The routine study of bone marrow trephine biopsies involves fixation, decalcification, paraffin-embedment, sectioning and staining. However, this process creates artifacts, produces shrinkage of tissue, consumes time and can result in sections of unsatisfactory cytological quality. It also renders the tissue unsuitable for enzyme-histochemical and immunohistochemical analyses. Frozen section of bone marrow without decalcification was evaluated as an alternative method for the study of bone marrow. This method was found to give sections with comparable cytological quality to that of paraffin-embedment, yielded sections for interpretation within 24 hours, and allowed enzyme-histochemical and immunohistochemical analyses to be applied successfully.
骨髓环钻活检的常规研究包括固定、脱钙、石蜡包埋、切片和染色。然而，这个过程会产生人为假象，导致组织收缩，耗费时间，并且可能得到细胞学质量不尽人意的切片。它还会使组织不适用于酶组织化学和免疫组织化学分析。未脱钙的骨髓冰冻切片被评估为一种研究骨髓的替代方法。结果发现，这种方法得到的切片在细胞学质量上与石蜡包埋相当，能在24小时内得到可供解读的切片，并且能成功应用酶组织化学和免疫组织化学分析。
