Schondelmeyer Curtis W, Dillehay Dirck L, Webb Sonji K, Huerkamp Michael J, Mook Deborah M, Pullium Jennifer K
Division of Animal Resources, Whitehead Biomedical Research Building, Emory University School of Medicine, Atlanta, Georgia, USA.
J Am Assoc Lab Anim Sci. 2006 Nov;45(6):40-3.
The Guide for the Care and Use of Laboratory Animals states that sanitization of caging accessories (for example, filter tops and wire-bar lids) should be done every 2 wk. In this study we tested the hypothesis that organic contamination measured by the presence of ATP associated with organic material (measured with luciferase test swabs) and the number of bacterial colony-forming units (as determined by use of replicate organism detection and counting plates) on caging accessories did not differ significantly at 2 wk versus several months of use. The study evaluated 4 groups: mouse and rat ventilated and static wire-bar cages with or without filter tops (n = 10 per group). The cages were evaluated at several time points from 2 wk to 6 mo. For every cage type, ATP levels did not differ significantly between 14 and 90 d and, in most cases, between 14 and 180 d. In addition the number of bacterial colonies did not differ significantly between 14 and 120 d (and, in some cases, between 14 and 180 d). This study provides data relevant to establishing a validated frequency for sanitization of rodent caging accessories while controlling, and potentially decreasing, costs associated with sanitization.
《实验动物饲养与使用指南》规定，笼具配件（如滤器顶盖和金属丝网盖）应每两周进行一次清洁消毒。在本研究中，我们检验了以下假设：通过与有机物质相关的ATP（用荧光素酶测试拭子测量）的存在来衡量的有机污染以及笼具配件上的细菌菌落形成单位数量（通过使用重复生物体检测和计数平板确定）在使用两周与使用几个月时并无显著差异。该研究评估了4组：有或无滤器顶盖的小鼠和大鼠通气及静态金属丝网笼（每组n = 10）。在从两周到6个月的多个时间点对笼子进行了评估。对于每种笼型，ATP水平在14天和90天之间以及在大多数情况下在14天和180天之间并无显著差异。此外，细菌菌落数量在14天和120天之间（以及在某些情况下在14天和180天之间）并无显著差异。本研究提供了相关数据，有助于确定对啮齿动物笼具配件进行有效清洁消毒的频率，同时控制并可能降低与清洁消毒相关的成本。
