Authier Simon, Chaurand Fernando, Legaspi Margarita, Breault Catherine, Troncy Eric
LAB Research INC., Laval, Quebec, Canada.
J Am Assoc Lab Anim Sci. 2006 Nov;45(6):73-9.
The purpose of this study was to evaluate 3 anesthetic protocols for intraduodenal drug administration by endoscopy in rhesus monkeys (Macaca mulatta). Anesthesia was induced using intramuscular ketamine and midazolam, isoflurane (inhalant gas), or intravenous propofol in male and female rhesus monkeys. A noninvasive dosing line was placed in the duodenum by use of endoscopy, and 50% dextrose (3 ml/kg) was administered. Blood pressure, heart rate, body temperature, and reflexes (corneal, palpebral, pharyngeal) and myorelaxation (mandibular reflex and reaction to limb manipulation) were evaluated every 5 min. To estimate intestinal absorption, glycemia was evaluated prior to dextrose administration and at 2, 5, 10, 15, 20, 30, 45, and 60 min after dosing. All 3 protocols resulted in successful induction of anesthesia. Recovery from isoflurane and propofol was significantly faster than from ketamine-midazolam. Duration of the recovery period after isoflurane was less variable than with propofol, but isoflurane produced greater hypothermia. Isoflurane and propofol resulted in predictable glucose absorption after intraduodenal dextrose administration, whereas ketamine-midazolam led to an inconsistent increase in glycemia.
本研究的目的是评估恒河猴（猕猴）经内镜十二指肠给药的三种麻醉方案。对雄性和雌性恒河猴使用肌肉注射氯胺酮和咪达唑仑、异氟烷（吸入性气体）或静脉注射丙泊酚诱导麻醉。通过内镜在十二指肠放置一条无创给药线，并给予50%葡萄糖（3 ml/kg）。每5分钟评估一次血压、心率、体温、反射（角膜反射、眼睑反射、咽反射）和肌松（下颌反射和对肢体操作的反应）。为评估肠道吸收情况，在给予葡萄糖前以及给药后2、5、10、15、20、30、45和60分钟评估血糖水平。所有三种方案均成功诱导了麻醉。异氟烷和丙泊酚麻醉后的苏醒明显快于氯胺酮-咪达唑仑麻醉。异氟烷麻醉后恢复期的时长比丙泊酚麻醉后的更稳定，但异氟烷导致的体温过低更严重。十二指肠给予葡萄糖后，异氟烷和丙泊酚麻醉可使葡萄糖吸收情况可预测，而氯胺酮-咪达唑仑麻醉则导致血糖水平的升高不稳定。
