Sharma Gangavaram V M, Jadhav Vivekanand B, Ramakrishna Kallaganti V S, Jayaprakash Pagadala, Narsimulu Kongari, Subash Velaparthi, Kunwar Ajit C
Organic Chemistry Division III, D-211, Discovery Laboratory, and NMR Group, Indian Institute of Chemical Technology, Hyderabad 500,007, India.
J Am Chem Soc. 2006 Nov 15;128(45):14657-68. doi: 10.1021/ja064875a.
New classes of alpha/gamma- and beta/gamma-hybrid peptides have been synthesized with novel 12/10- and 11/13-mixed helical patterns, respectively. The alpha/gamma-peptides were derived from the dipeptide repeats with alternating arrays of l-Ala and gamma-Caa((l)) (C-linked carbo-gamma-amino acid from d-mannose), which generated a new 12/10-mixed helix, for the first time, without a beta-amino acid. The beta/gamma-peptides made from an alternating arrangement of beta-Caa((x)) (C-linked carbo-beta-amino acid) and gamma-Caa((x)) (C-linked carbo-gamma-amino acid from d-xylose), on the other hand, resulted in an unprecedented 11/13-helix. The secondary structures in these peptides have been ascertained from detailed NMR studies, and CD spectroscopy and molecular dynamics investigations provided additional support for the structures derived.
已分别合成了具有新型12/10和11/13混合螺旋模式的α/γ-和β/γ-杂交肽新类别。α/γ-肽源自具有l-Ala和γ-Caa((l))（来自d-甘露糖的C-连接的碳-γ-氨基酸）交替排列的二肽重复序列，首次产生了一种新的12/10混合螺旋，且不含β-氨基酸。另一方面，由β-Caa((x))（C-连接的碳-β-氨基酸）和γ-Caa((x))（来自d-木糖的C-连接的碳-γ-氨基酸）交替排列制成的β/γ-肽产生了前所未有的11/13螺旋。这些肽的二级结构已通过详细的核磁共振研究确定，圆二色光谱和分子动力学研究为推导得到的结构提供了额外支持。
