Fang Zhi-Hong, Cui Jian-Wei, Cao Jian-Mei, Peng Jing-Hua, Feng Qin, Hu Yi-Yang
Institute of Liver Diseases, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Zhong Xi Yi Jie He Xue Bao. 2006 Nov;4(6):596-600. doi: 10.3736/jcim20060611.
Duplicating the classical alcoholic hepatic injury model, to provide an ideal animal model for research on prevention and treatment of hepatic injury.
According to the prescription of Lieber-DeCarli, the same calorie fluid animal feed, which contained ethanol or non-ethanol, was prepared. Twenty-three rats were divided into normal control group (n=5), control liquid diet group (n=9), ethanol liquid diet group (n=9). Rats in the normal control group were fed normal diet, and rats in the control liquid diet group and ethanol liquid diet group were fed the corresponding diet for eight weeks. The pathologic changes of hepatic tissue were observed. The activities of the serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST), the liver tissue gamma-glutamyltransferase (gamma-GT), the content of triglyceride (TG), and the lipid peroxidation by-products were assayed.
Compared to the normal control and the control liquid diet groups, the activities of ALT, AST, and gamma-GT, and the lipid peroxidation by-products increased significantly in the ethanol liquid diet group. The pathological changes of cellular swelling and fatty degeneration in the ethanol liquid diet group were severe.
Alcoholic hepatic injury model can be successfully duplicated by Lieber-DeCarli prescription.
复制经典酒精性肝损伤模型，为肝损伤防治研究提供理想的动物模型。
按照Lieber-DeCarli配方，制备含乙醇或不含乙醇的等热量液体动物饲料。将23只大鼠分为正常对照组（n = 5）、对照液体饲料组（n = 9）、乙醇液体饲料组（n = 9）。正常对照组大鼠喂以正常饮食，对照液体饲料组和乙醇液体饲料组大鼠分别喂以相应饲料8周。观察肝组织病理变化。检测血清丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）活性，肝组织γ-谷氨酰转移酶（γ-GT）、甘油三酯（TG）含量及脂质过氧化产物。
与正常对照组和对照液体饲料组相比，乙醇液体饲料组ALT、AST、γ-GT活性及脂质过氧化产物显著增加。乙醇液体饲料组细胞肿胀和脂肪变性等病理变化严重。
采用Lieber-DeCarli配方可成功复制酒精性肝损伤模型。
