School of Nutrition and Health Sciences, College of Public Health & Nutrition, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, Taiwan.
Alcohol. 2013 Sep;47(6):467-72. doi: 10.1016/j.alcohol.2013.05.006. Epub 2013 Jul 2.
The purpose of this study was to investigate the protective effects of nanogold flakes against alcoholic liver disease. Six-week-old male Wistar rats were divided into 6 groups: C (control liquid diet), CLF (control liquid diet with gold flakes at 1.03 mg/kg body weight [BW]/day), CHF (control liquid diet with gold flakes at 5.15 mg/kg BW/day), E (ethanol liquid diet), ELF (ethanol liquid diet with gold flakes at 1.03 mg/kg BW/day), and EHF (ethanol liquid diet with gold flakes at 5.15 mg/kg BW/day). The liquid diets were prepared daily. Gold flakes were added to the ethanol 1 h before preparing the ethanol liquid diets, as an aging process. After 10 weeks, rats in group E showed significantly higher plasma aspartate transaminase (AST) and alanine transaminase (ALT) activities than those in group C. A significantly increased concentration of hepatic triglyceride (TG) was found in group E. Furthermore, higher hepatic glutathione reductase (GRD), superoxide dismutase (SOD), and catalase (CAT) activities together with higher tumor necrosis factor (TNF)-α concentration and higher hepatic cytochrome (CYP2E1) protein expression were also observed in group E. In contrast, the hepatic TG concentration in group EHF was significantly lower than that of group E. In addition, hepatic glutathione peroxidase (GPX), SOD, and CAT activities together with TNF-α concentration and hepatic CYP2E1 protein expression in group EHF were significantly lower than those in group E. We concluded that nanogold flakes might ameliorate alcohol-induced liver injury by maintaining the hepatic antioxidative status. In addition, nanogold flakes may reduce fat accumulation caused by chronic ethanol feeding via decreasing hepatic TNF-α.
本研究旨在探讨纳米金片对酒精性肝病的保护作用。将 6 周龄雄性 Wistar 大鼠分为 6 组：C（对照液体饮食）、CLF（对照液体饮食加金片 1.03mg/kg 体重/天）、CHF（对照液体饮食加金片 5.15mg/kg 体重/天）、E（乙醇液体饮食）、ELF（含金片 1.03mg/kg 体重/天的乙醇液体饮食）和 EHF（含金片 5.15mg/kg 体重/天的乙醇液体饮食）。每天准备液体饮食。在制备乙醇液体饮食前 1 小时向乙醇中加入金片，作为老化过程。10 周后，与 C 组相比，E 组大鼠血浆天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）活性明显升高。E 组大鼠肝甘油三酯（TG）浓度明显升高。此外，E 组大鼠肝还原型谷胱甘肽（GRD）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性升高，肿瘤坏死因子（TNF）-α浓度升高，肝细胞色素（CYP2E1）蛋白表达升高。相反，EHF 组的肝 TG 浓度明显低于 E 组。此外，EHF 组大鼠肝谷胱甘肽过氧化物酶（GPX）、SOD 和 CAT 活性以及 TNF-α浓度和肝 CYP2E1 蛋白表达均明显低于 E 组。我们得出结论，纳米金片可能通过维持肝抗氧化状态来改善酒精引起的肝损伤。此外，纳米金片可能通过减少肝 TNF-α来减少慢性乙醇喂养引起的脂肪堆积。
