Li Huanzhou, Qiu Ping, Wang Juanhong, Niu Congcong, Pan Suhua
College of Pharmacy, Nanjing University of Chinese Medicine, 138 Xianlin Road, Qixia District, Nanjing 210023, P. R. China.
Food Funct. 2015 Feb;6(2):470-8. doi: 10.1039/c4fo00739e.
This study aimed to investigate the effects of Compound Ginkgo biloba (CGB) on alterations in intestinal permeability and inflammation caused by endotoxin in chronic alcohol-induced liver injury. CGB was prepared by Ginkgo biloba extract and Rosa roxburghii in a 1 : 1 proportion. Rats were divided into four groups: control, ethanol, high-dosage CGB (0.6 g kg(-1) d(-1)) and low-dosage CGB (0.2 g kg(-1) d(-1)) group. Rats in the control group ingested a Lieber-DeCarli control liquid diet, while rats in the ethanol and CGB-treated groups ingested a Lieber-DeCarli alcohol liquid diet for eight weeks. CGB was orally administered from the beginning of the third week until the end of the experiment. CGB was observed to significantly reduce the activities of serum ALT, AST, diamine oxidase (DAO) as well as levels of serum TG, D-lactic acid and plasma endotoxin in rats fed with Lieber-DeCarli ethanol liquid. Further, the hepatic steatosis was improved and the damage to intestinal tight junctions was also relieved effectively after CGB administration. Moreover, CGB significantly downregulated the expressions of TNF-α, lipopolysaccharide binding protein (LBP), CD14 and TLR4 in the liver and upregulated the expressions of tight junction proteins including ZO-1, occludin and claudin-1. In summary, this study demonstrated that CGB alleviated alcohol-induced liver injury and hepatic lipopolysaccharide signaling as well as gut barrier dysfunction through restoring tight junctions.
本研究旨在探讨复方银杏叶（CGB）对慢性酒精性肝损伤中内毒素所致肠道通透性改变和炎症的影响。CGB由银杏叶提取物和刺梨按1∶1比例制备而成。将大鼠分为四组：对照组、乙醇组、高剂量CGB组（0.6 g·kg⁻¹·d⁻¹）和低剂量CGB组（0.2 g·kg⁻¹·d⁻¹）。对照组大鼠摄入Lieber-DeCarli对照液体饲料，而乙醇组和CGB处理组大鼠摄入Lieber-DeCarli酒精液体饲料，持续8周。从第三周开始至实验结束，对大鼠进行CGB灌胃。结果观察到，CGB能显著降低摄入Lieber-DeCarli乙醇液体饲料大鼠的血清谷丙转氨酶（ALT）、谷草转氨酶（AST）、二胺氧化酶（DAO）活性以及血清甘油三酯（TG）、D-乳酸水平和血浆内毒素水平。此外，给予CGB后，肝脂肪变性得到改善，肠道紧密连接损伤也得到有效缓解。而且CGB显著下调肝脏中肿瘤坏死因子-α（TNF-α）、脂多糖结合蛋白（LBP）、CD14和Toll样受体4（TLR4）的表达，并上调包括紧密连接蛋白1（ZO-1）、闭合蛋白（occludin）和紧密连接蛋白1（claudin-1）在内的紧密连接蛋白表达。综上所述，本研究表明CGB通过恢复紧密连接减轻了酒精性肝损伤、肝脏脂多糖信号转导以及肠道屏障功能障碍。
