Martins da Costa Paulo M, Vaz-Pires Paulo M, Bernardo Fernando M
ICBAS - Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, Porto, Portugal.
J Environ Sci Health B. 2006;41(8):1393-403. doi: 10.1080/03601230600964258.
Antibiotimicrobial resistance was investigated in 537 Enterococcus spp. isolates recovered from 22 samples of crude inflow, treated effluent and sludge collected in wastewater treatment plants of eight poultry slaughterhouses of Portugal. No significant differences (P > 0.05) were found in the resistance to each antimicrobial agent with regards to the origin of the sample (inflow, sludge and effluent). Many of the isolates displayed resistance to tetracycline (85.7%), erythromycin (45.7%), nitrofurantoin (34.0%) and rifampicin (17.8%). Resistance was also observed, but to a lesser extent, to ciprofloxacin (10.2%), ampicillin (8.0%), chloramphenicol (4.6%), vancomycin (0.9%) and gentamicin (0.4%). Resistance to three or more antimicrobial classes was present in 37.1% of the isolates. Wastewater treatment resulted in viable enterococci decrease between less than 1 log and 4 log; nevertheless, more than 4.4 x 10(5) colony forming units (CFU)/100 mL were present in the outflow of the plants and thus resistant enterococci are not prevented from reaching the general environment.
对从葡萄牙8家家禽屠宰场的污水处理厂采集的22份原水、处理后的废水和污泥样本中分离出的537株肠球菌进行了抗生素耐药性调查。就样本来源（原水、污泥和废水）而言，对每种抗菌剂的耐药性未发现显著差异（P>0.05）。许多分离株对四环素（85.7%）、红霉素（45.7%）、呋喃妥因（34.0%）和利福平（17.8%）表现出耐药性。对环丙沙星（10.2%）、氨苄西林（8.0%）、氯霉素（4.6%）、万古霉素（0.9%）和庆大霉素（0.4%）也观察到耐药性，但程度较轻。37.1%的分离株对三种或更多抗菌类别具有耐药性。废水处理使存活的肠球菌减少了不到1个对数至4个对数；然而，工厂流出物中每100 mL仍存在超过4.4×10⁵菌落形成单位（CFU），因此耐药肠球菌无法被阻止进入一般环境。
