Wilhelmsson Ulrika, Bushong Eric A, Price Diana L, Smarr Benjamin L, Phung Van, Terada Masako, Ellisman Mark H, Pekny Milos
Department of Clinical Neuroscience and Rehabilitation, Institute of Neuroscience and Physiology, Sahlgrenska Academy, Göteborg University, SE-405 30 Göteborg, Sweden.
Proc Natl Acad Sci U S A. 2006 Nov 14;103(46):17513-8. doi: 10.1073/pnas.0602841103. Epub 2006 Nov 7.
Reactive astrocytes in neurotrauma, stroke, or neurodegeneration are thought to undergo cellular hypertrophy, based on their morphological appearance revealed by immunohistochemical detection of glial fibrillary acidic protein, vimentin, or nestin, all of them forming intermediate filaments, a part of the cytoskeleton. Here, we used a recently established dye-filling method to reveal the full three-dimensional shape of astrocytes assessing the morphology of reactive astrocytes in two neurotrauma models. Both in the denervated hippocampal region and the lesioned cerebral cortex, reactive astrocytes increased the thickness of their main cellular processes but did not extend to occupy a greater volume of tissue than nonreactive astrocytes. Despite this hypertrophy of glial fibrillary acidic protein-containing cellular processes, interdigitation between adjacent hippocampal astrocytes remained minimal. This work helps to redefine the century-old concept of hypertrophy of reactive astrocytes.
基于通过免疫组织化学检测胶质纤维酸性蛋白、波形蛋白或巢蛋白所揭示的形态外观，神经创伤、中风或神经退行性疾病中的反应性星形胶质细胞被认为会发生细胞肥大，所有这些蛋白都会形成中间丝，即细胞骨架的一部分。在此，我们使用一种最近建立的染料填充方法来揭示星形胶质细胞的完整三维形状，评估两种神经创伤模型中反应性星形胶质细胞的形态。在去神经支配的海马区和受损的大脑皮层中，反应性星形胶质细胞均增加了其主要细胞突起的厚度，但与非反应性星形胶质细胞相比，并未扩展到占据更大的组织体积。尽管含有胶质纤维酸性蛋白的细胞突起出现这种肥大，但相邻海马星形胶质细胞之间的相互交错仍然很少。这项工作有助于重新定义已有百年历史的反应性星形胶质细胞肥大的概念。
