Ikuta Shuzo, Sekino Nobufumi, Hara Takeshi, Saito Yuriko, Chida Kazuhiro
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Biosci Biotechnol Biochem. 2006 Nov;70(11):2669-75. doi: 10.1271/bbb.60266. Epub 2006 Nov 7.
We describe an organotypic model of mouse skin consisting of a stratified sheet of epidermal keratinocytes and dermal fibroblasts within a contracted collagen gel. The model was designed to maintain the polarity of stratified keratinocytes and permit their long-term culture at an air-liquid interface. After air exposure, the thickness of the keratinocyte sheet transiently increased and then decreased to two cell layers at 2 weeks. The two-cell-layer structure is similar to that of the adult mouse epidermis. Cytokeratin 5 was localized in the lowest cell layer in the epithelial sheet, but cytokeratin 1 and loricrin were localized in the outer cell layers, resembling mouse skin. The expressions of interleukin 1alpha and 1beta in the keratinocytes and of keratinocyte growth factor 1 and 2 in the fibroblasts correlated with keratinocyte stratification. The mouse organotypic coculture is useful in studying epithelial cell-mesenchymal cell interactions in vitro.
我们描述了一种小鼠皮肤的器官型模型，该模型由收缩胶原凝胶内的分层表皮角质形成细胞片层和真皮成纤维细胞组成。该模型旨在维持分层角质形成细胞的极性，并允许它们在气液界面进行长期培养。暴露于空气中后，角质形成细胞片层的厚度短暂增加，然后在2周时降至两层细胞。这种两层细胞结构类似于成年小鼠表皮。细胞角蛋白5定位于上皮片层的最底层细胞，但细胞角蛋白1和兜甲蛋白定位于外层细胞，类似于小鼠皮肤。角质形成细胞中白细胞介素1α和1β以及成纤维细胞中角质形成细胞生长因子1和2的表达与角质形成细胞分层相关。小鼠器官型共培养在体外研究上皮细胞-间充质细胞相互作用方面很有用。
