Galiana E, Vernier P, Dupont E, Evrard C, Rouget P
Laboratoire Biologie Moléculaire et Différenciation, Université Paris-6, Collège de France.
Proc Natl Acad Sci U S A. 1995 Feb 28;92(5):1560-4. doi: 10.1073/pnas.92.5.1560.
Immortalized neural precursor cell lines carrying the polyoma large tumor (T) gene have been shown previously to retain a clear-cut contact inhibition of growth and to differentiate in vitro. In the present study, we have identified and isolated cDNA clones corresponding to RNA expressed preferentially when these cells reach confluence. One of them, NPDC-1, is expressed specifically in the nervous system. The transfection of dividing cells with a NPDC-1 expression vector results in the inhibition of cell proliferation. In addition, the stable introduction of NPDC-1 into transformed cells, even of nonneural origin, leads to the suppression of transformed characteristics.
先前已表明，携带多瘤大肿瘤（T）基因的永生化神经前体细胞系在体外能保持明显的生长接触抑制并发生分化。在本研究中，我们鉴定并分离出了与这些细胞达到汇合时优先表达的RNA相对应的cDNA克隆。其中一个克隆NPDC-1在神经系统中特异性表达。用NPDC-1表达载体转染分裂细胞会导致细胞增殖受到抑制。此外，将NPDC-1稳定导入转化细胞（甚至是非神经来源的细胞）会导致转化特征受到抑制。
