Cameron Brent D, Li Yanfang, Nezhuvingal Ajaina
Department of Bioengineering, University of Toledo, Toledo, Ohio 43606-3390, USA.
J Biomed Opt. 2006 Sep-Oct;11(5):054031. doi: 10.1117/1.2363347.
A need exists for the continued development of diagnostic tools and methods capable of distinguishing and characterizing slight differences in the optical properties of tissues. We present a method to estimate the scattering coefficient contribution as a function of particle size in complex mixtures of polystyrene spheres. The experimental method we used is a Mueller matrix imaging approach. The Mueller matrix encodes the polarization-dependent properties of the sample and describes how a given sample will transform an incident light polarization state. A partial least-squares approach is used to form a model around a set of Mueller matrix image-based measurements to accurately predict the individual scattering coefficient contributions in phantoms containing 0.2, 0.5, 1, and 2 microm-diameter polystyrene spheres. The results show individual scattering coefficient contribution errors as low as 0.1585 cm(-1) can be achieved. In addition, it is shown how the scattering type (i.e., Rayleigh and Mie) is encoded within the Mueller matrix. Such methods may eventually lead to the development of improved diagnostic tools capable of characterizing and distinguishing between tissue abnormalities, such as superficial cancerous lesions from their benign counterparts.
持续开发能够区分和表征组织光学特性细微差异的诊断工具和方法很有必要。我们提出了一种方法，用于估计聚苯乙烯球体复杂混合物中散射系数随粒径的贡献。我们使用的实验方法是穆勒矩阵成像方法。穆勒矩阵对样品的偏振相关特性进行编码，并描述给定样品将如何改变入射光的偏振态。使用偏最小二乘法围绕一组基于穆勒矩阵图像的测量值构建模型，以准确预测包含直径为0.2、0.5、1和2微米的聚苯乙烯球体的体模中各个散射系数的贡献。结果表明，单个散射系数贡献误差可低至0.1585 cm⁻¹。此外，还展示了散射类型（即瑞利散射和米氏散射）如何在穆勒矩阵中编码。此类方法最终可能会促成改进的诊断工具的开发，这些工具能够表征和区分组织异常，例如浅表癌性病变与其良性对应物。
