Morakotkarn Doungporn, Kawasaki Hiroko, Seki Tatsuji
The International Center for Biotechnology, Osaka University, Osaka, Japan.
FEMS Microbiol Lett. 2007 Jan;266(1):10-9. doi: 10.1111/j.1574-6968.2006.00489.x. Epub 2006 Nov 1.
Bamboos are common and useful plants in Japan but little information is available about their endophytes. In this study, 257 fungal strains were isolated from bamboo tissues, and 71 representative strains were characterized by 18S rRNA gene and internal transcriber spacer region sequence analysis. Phylogenetic analysis showed that the fungal isolates were located in Sordariomycetes and Dothideomycetes. Xylariales was the dominant group within bamboos. Several rRNA gene sequences were not similar to any current sequence in the database and might be a novel species or genera that could represent sources of novel biological compounds. These findings reveal that bamboos are a huge reservoir of microorganisms that should be extensively investigated.
竹子在日本是常见且有用的植物,但关于其内生菌的信息却很少。在本研究中,从竹子组织中分离出257株真菌菌株,并通过18S rRNA基因和内部转录间隔区序列分析对71株代表性菌株进行了表征。系统发育分析表明,分离出的真菌位于粪壳菌纲和座囊菌纲。炭角菌目是竹子中的优势类群。一些rRNA基因序列与数据库中目前的任何序列都不相似,可能是新物种或新属,可能代表新型生物化合物的来源。这些发现表明,竹子是一个巨大的微生物库,值得深入研究。