Microbiology Laboratory, Nanjing Municipal Center for Disease Control and Prevention, Nanjing, 210003, People's Republic of China.
College of Life Sciences, Nanjing Normal University, No.1 Wenyuan Road, Qixia District, Nanjing, 210023, People's Republic of China.
Sci Rep. 2021 Jan 27;11(1):2365. doi: 10.1038/s41598-021-81990-2.
Shiraia bambusicola has been used as a traditional Chinese medicine for a long history. Its major medicinal active metabolites are perylenequinones, including hypocrellin A, elsinochrome A and so on. At present, the fermentation yield of perylenequinones is low, and its complex biosynthesis and regulatory pathways are still unclear. In this study, nitric oxide, as a downstream signal molecule of hydrogen peroxide, regulates the biosynthesis of perylenequinones. Exogenous addition of 0.01 mM sodium nitroprusside (nitric oxide donor) can promote perylenequinones production by 156% compared with the control. Further research found that hydrogen peroxide and nitric oxide increased the transcriptional level of the biosynthetic genes of hypocrellin A. The results showed that nitric oxide is involved in the biosynthesis and regulation of perylenequinones in Shiraia bambusicola as a signal molecule. In the future, the yield of perylenequinones can be increased by adding exogenous nitric oxide in fermentation.
竹红菌已被作为一种传统的中药使用了很长的历史。它的主要药用活性代谢产物是二萘嵌苯醌，包括竹红菌素 A、表鬼臼毒素 A 等。目前，二萘嵌苯醌的发酵产量较低，其复杂的生物合成和调控途径尚不清楚。在本研究中，一氧化氮作为过氧化氢的下游信号分子，调节二萘嵌苯醌的生物合成。与对照组相比，外源添加 0.01 mM 硝普钠（一氧化氮供体）可使二萘嵌苯醌产量提高 156%。进一步的研究发现，过氧化氢和一氧化氮增加了竹红菌素 A 生物合成基因的转录水平。结果表明，一氧化氮作为信号分子参与竹红菌中二萘嵌苯醌的生物合成和调控。未来可以通过在发酵过程中外源添加一氧化氮来提高二萘嵌苯醌的产量。
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