Yigla Mordechai, Berkovich Yaron, Nagler Rafael M
Pulmunology Department, Rambam Medical Center and Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa 31096, Israel.
Arch Oral Biol. 2007 Jan;52(1):36-43. doi: 10.1016/j.archoralbio.2006.08.002. Epub 2006 Nov 7.
It has been suggested that oxidative stress plays a role in the pathogenesis of chronic obstructive pulmonary disease (COPD), though this role has yet to be fully elucidated. The purpose of this study was to further evaluate this role as concomitantly expressed in the saliva and broncho-alveolar lavage (BAL/'lavage').
Forty consenting patients (mean age 62+/-13-year-old), with/without COPD and/or smoking habit, participated in the study. The following antioxidant profile was examined both in saliva and lavage of the patients: total antioxidant status (TAS), uric acid (UA), peroxidase and super oxide dismutase (SOD). Total protein (TP) and albumin (Alb) were also evaluated in both saliva and lavage while amylase was measured only in saliva.
Increase of TAS (by 100%) and of SOD activity levels (by 60%) in the lavage of COPD patients indicated oxidative stress. The salivary UA in COPD patients was 125% higher (p = 0.05) while the peroxidase was 20% higher. Another novel finding was that levels of salivary antioxidants in smoking versus non-smoking COPD patients were lower by 25-48% (for all four: TAS, UA, peroxidase and SOD) while the albumin was significantly reduced by 60% (p = 0.018).
Oxidative-stress-related changes demonstrated both in the lavage and saliva of the COPD and/or smoking patient indicate cumulative effects of both, also emphasizing the pathogenetic role of free radicals in COPD. Salivary analysis, which is less invasive and much easier to perform as compared with lavage analysis, is suggested as a new and effective diagnostic tool in COPD patients.
尽管氧化应激在慢性阻塞性肺疾病（COPD）发病机制中的作用尚未完全阐明，但已有研究表明其发挥了一定作用。本研究的目的是进一步评估氧化应激在唾液和支气管肺泡灌洗（BAL/灌洗）中同时表达时所起的作用。
40名自愿参与的患者（平均年龄62±13岁），有或无COPD及/或吸烟习惯，参与了本研究。对患者的唾液和灌洗液检测了以下抗氧化指标：总抗氧化状态（TAS）、尿酸（UA）、过氧化物酶和超氧化物歧化酶（SOD）。同时还评估了唾液和灌洗液中的总蛋白（TP）和白蛋白（Alb），而淀粉酶仅在唾液中进行测定。
COPD患者灌洗液中TAS升高（100%）以及SOD活性水平升高（60%）表明存在氧化应激。COPD患者唾液中的UA升高了125%（p = 0.05），而过氧化物酶升高了20%。另一个新发现是，吸烟的COPD患者与不吸烟的COPD患者相比，唾液抗氧化剂水平降低了25 - 48%（TAS、UA、过氧化物酶和SOD这四项指标均如此），而白蛋白显著降低了60%（p = 0.018）。
在COPD和/或吸烟患者的灌洗液和唾液中均显示出与氧化应激相关的变化，这表明两者都有累积效应，同时也强调了自由基在COPD发病机制中的作用。与灌洗分析相比，唾液分析侵入性较小且更易于操作，建议将其作为COPD患者一种新的有效诊断工具。
