Ben-Zvi Ilan, Green Yaakov, Nakhoul Farid, Kanter Yoram, Nagler Rafael M
Nephrology Department, Rambam Medical Center and Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel.
Nephron Clin Pract. 2007;105(3):c114-20. doi: 10.1159/000098320. Epub 2006 Dec 29.
To analyze various oxidative stress parameters in the saliva and serum of patients with chronic renal failure (CRF) and/or diabetes mellitus (DM), and to compare them in dialytic vs. pre-dialytic patients.
50 consenting patients were divided into five subgroups of patients: severe CRF (dialytic) without DM, severe CRF (dialytic) with DM, mild CRF (pre-dialytic) without DM, mild CRF (pre-dialytic) with DM, and with DM but without CRF (controls). Uric acid (UA), peroxidase and total antioxidant status (TAS) were studied in both saliva and serum; superoxide dismutase (SOD) was evaluated only in saliva. Both saliva collection and serum harvesting were done simultaneously.
In severe-CRF patients without DM, median TAS, UA and SOD levels decreased following dialysis (54, 85, 48%, respectively), and peroxidase levels increased slightly (9%). In severe-CRF patients with DM, median TAS and SOD levels increased following dialysis (33 and 54%, respectively) while median UA and peroxidase levels decreased (68 and 10%, respectively).
DM, CRF and hemodialysis were found to increase the oxidative stress burden in both serum and saliva. Therefore, antioxidant assessment may be used to monitor baseline oxidative status in these situations though larger randomized studies are in order.
分析慢性肾衰竭（CRF）和/或糖尿病（DM）患者唾液和血清中的各种氧化应激参数，并比较透析患者与透析前患者的这些参数。
50名同意参与的患者被分为五个亚组：无DM的重度CRF（透析）患者、有DM的重度CRF（透析）患者、无DM的轻度CRF（透析前）患者、有DM的轻度CRF（透析前）患者以及有DM但无CRF的患者（对照组）。对唾液和血清中的尿酸（UA）、过氧化物酶和总抗氧化状态（TAS）进行了研究；仅在唾液中评估了超氧化物歧化酶（SOD）。唾液采集和血清采集同时进行。
在无DM的重度CRF患者中，透析后TAS、UA和SOD水平的中位数下降（分别为54%、85%、48%），而过氧化物酶水平略有上升（9%）。在有DM的重度CRF患者中，透析后TAS和SOD水平的中位数上升（分别为33%和54%），而UA和过氧化物酶水平的中位数下降（分别为68%和10%）。
发现DM、CRF和血液透析会增加血清和唾液中的氧化应激负担。因此，尽管需要进行更大规模的随机研究，但抗氧化评估可用于监测这些情况下的基线氧化状态。
