Tan Wenbin, Sun Lixin, Zhang Donghui, Sun Jing, Qian Jin, Hu Xiaobang, Wang Weijie, Sun Yan, Ma Lei, Zhu Changliang
Department of Pathogenic Biology, Nanjing Medical University, Nanjing, Jiangsu Province, PR China.
Exp Parasitol. 2007 Apr;115(4):369-78. doi: 10.1016/j.exppara.2006.09.020. Epub 2006 Nov 7.
The complete sequence of ribosomal protein L39 has been cloned from deltamethrin-resistant strain of Culex pipiens pallens (DR1 strain). Quantitative RT-PCR analysis indicated that the RPL39 transcription level was 23.4 times higher in DR1 strain than in susceptible strain at 4th instar larvae. The RPL39 expression was also found to be consistently higher throughout the life cycle of DR1 strain. A protein of predicted size 17 kDa has been detected by Western blotting in RPL39-transfected mosquito C6/36 cells. These RPL39-transfected cells also showed enhanced deltamethrin resistance compared to plasmid vector-transfected cells as determined by methyl tritiated thymidine ((3)H-TdR) incorporation. These results indicate that RPL39 is expressed at higher levels in DR1 strain, and may confer some insecticide resistance in Cx. pipiens pallens.
从淡色库蚊溴氰菊酯抗性品系（DR1品系）中克隆出核糖体蛋白L39的完整序列。定量逆转录聚合酶链反应（qRT-PCR）分析表明，在四龄幼虫阶段，DR1品系中RPL39的转录水平比敏感品系高23.4倍。还发现RPL39在DR1品系的整个生命周期中表达始终较高。通过蛋白质免疫印迹法在转染RPL39的蚊虫C6/36细胞中检测到预测大小为17 kDa的蛋白质。通过甲基化氚标记胸腺嘧啶核苷（（3）H-TdR）掺入法测定，与转染质粒载体的细胞相比，这些转染RPL39的细胞对溴氰菊酯的抗性也增强。这些结果表明，RPL39在DR1品系中高水平表达，并且可能赋予淡色库蚊一定的抗药性。
