Hu Xiaobang, Wang Weijie, Zhang Donghui, Jiao Jianhua, Tan Wenbin, Sun Yan, Ma Lei, Zhu Changliang
Department of Pathogen Biology, Nanjing Medical University; Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, PR China.
Comp Biochem Physiol B Biochem Mol Biol. 2007 Feb;146(2):265-70. doi: 10.1016/j.cbpb.2006.11.011. Epub 2006 Nov 25.
The 40S ribosomal protein S4 gene (RPS4) has been cloned from Culex pipiens pallens. An open reading frame (ORF) of 789 bp was found to encode a putative 262 amino acid protein. The deduced amino acid sequence shares 96% and 91% identity with RPS4 genes from Aedes and Anopheles respectively. Transcript expression of RPS4 was determined by real-time PCR in all life stages of deltamethrin-susceptible and -resistant strains. The results demonstrated that this gene is expressed at all developmental stages. Meanwhile, in pupae and adults, RPS4 is overexpressed in deltamethrin-resistant strain than in -susceptible strain. Our data for the first time suggests that increased expression of the RPS4 gene may play some role in the development of deltamethrin resistance in C. pipiens pallens.
已从淡色库蚊中克隆出40S核糖体蛋白S4基因（RPS4）。发现一个789 bp的开放阅读框（ORF）编码一种推定的262个氨基酸的蛋白质。推导的氨基酸序列与伊蚊和按蚊的RPS4基因分别具有96%和91%的同一性。通过实时PCR测定了RPS4在溴氰菊酯敏感和抗性品系所有生命阶段的转录表达。结果表明该基因在所有发育阶段均有表达。同时，在蛹期和成虫期，RPS4在溴氰菊酯抗性品系中的表达高于敏感品系。我们的数据首次表明，RPS4基因表达的增加可能在淡色库蚊对溴氰菊酯抗性的形成中起一定作用。
