Halliday G, Flowers D, Baum L
Department of Pathology, University of Sydney, NSW, Australia.
Acta Neuropathol. 1994;87(2):174-86. doi: 10.1007/BF00296188.
This study evaluated current methods for demonstrating and categorizing cortical plaques, with the aim of establishing objective methodology for future diagnostic evaluation. Analysis of four methods of tissue processing revealed that the highest numbers of plaques were identified in formalin-fixed, paraffin-embedded tissue regardless of the stain used. Analysis of three silver stains and four immunohistochemical dilutions of an antibody to beta A4 protein revealed that the recent silver method published by Garvey et al. [(1990) J Histotechnol 14: 39-42] was equivalent to beta A4 immunohistochemistry in demonstrating the highest number of plaques. Plaque differentiation was easier and more reliable in silver compared to beta A4-stained sections, although the number of identifiable small compact plaques was significantly reduced in silver-stained sections. These studies show that plaque differentiation may be compromised by tissue processing and staining protocols. The establishment of superior methods may provide better diagnostic resolution for patients with Alzheimer's disease.
本研究评估了当前用于显示和分类皮质斑块的方法，目的是为未来的诊断评估建立客观的方法。对四种组织处理方法的分析表明，无论使用何种染色剂，在福尔马林固定、石蜡包埋的组织中发现的斑块数量最多。对三种银染法和针对β - A4蛋白的抗体的四种免疫组化稀释度的分析表明，Garvey等人[（1990年）《组织技术杂志》14: 39 - 42]最近发表的银染法在显示最多数量的斑块方面与β - A4免疫组化相当。与β - A4染色切片相比，银染切片中斑块的区分更容易且更可靠，尽管在银染切片中可识别的小致密斑块数量显著减少。这些研究表明，斑块的区分可能会受到组织处理和染色方案的影响。建立更优的方法可能为阿尔茨海默病患者提供更好的诊断分辨率。
